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DOC2A Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - DOC2A Antibody (Center) AP12839c
    DOC2A Antibody (Center) (Cat. #AP12839c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
  • WB - DOC2A Antibody (Center) AP12839c
    DOC2A Antibody (Center) (Cat. #AP12839c) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DOC2A antibody detected the DOC2A protein (arrow).
  • IHC-P - DOC2A Antibody (Center) AP12839c
    DOC2A Antibody (Center) (Cat. #AP12839c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DOC2A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, WB, IHC-P, E
Primary Accession Q14183
Other Accession Q14184, P70611, Q7TNF0, NP_003577.2
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 43959 Da
Antigen Region 277-306 aa
Additional Information
Gene ID 8448
Other Names Double C2-like domain-containing protein alpha, Doc2, Doc2-alpha, DOC2A
Target/Specificity This DOC2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the Central region of human DOC2A.
Dilution FC~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDOC2A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DOC2A
Function Calcium sensor which most probably regulates fusion of vesicles with membranes. Binds calcium and phospholipids. May be involved in calcium dependent neurotransmitter release through the interaction with UNC13A. May be involved in calcium-dependent spontaneous release of neurotransmitter in absence of action potentials in neuronal cells. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.
Cellular Location Lysosome. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome. Note=Colocalizes to synaptic vesicles
Tissue Location Predominantly expressed in brain. Also expressed in testis.
Citations (0)

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Background

There are at least two protein isoforms of the Double C2 protein, namely alpha (DOC2A) and beta (DOC2B), which contain two C2-like domains. DOC2A and DOC2B are encoded by different genes; these genes are at times confused with the unrelated DAB2 gene which was initially named DOC-2. DOC2A is mainly expressed in brain and is suggested to be involved in Ca(2+)-dependent neurotransmitter release.

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Glessner, J.T., et al. Proc. Natl. Acad. Sci. U.S.A. 107(23):10584-10589(2010)
Guilmatre, A., et al. Arch. Gen. Psychiatry 66(9):947-956(2009)
Kumar, R.A., et al. PLoS ONE 4 (2), E4582 (2009) :
Higashio, H., et al. J. Immunol. 180(7):4774-4784(2008)

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$ 295.00
$ 99.00
Cat# AP12839c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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