|Application ||WB, IHC-P, E|
|Calculated MW||49976 Da|
|Antigen Region||228-257 aa|
|Other Names||GDP-fucose protein O-fucosyltransferase 2, Peptide-O-fucosyltransferase 2, O-FucT-2, POFUT2, C21orf80, FUT13, KIAA0958|
|Target/Specificity||This POFUT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the Central region of human POFUT2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||POFUT2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||C21orf80, FUT13, KIAA0958|
|Function||Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type 1 repeats where C1 and C2 are the first and second cysteines, respectively. O-fucosylates members of several protein families including the ADAMTS family, the thrombosporin (TSP) and spondin families. The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm (By similarity). Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13.|
|Cellular Location||Endoplasmic reticulum. Golgi apparatus. Note=Mainly located in the endoplasmic reticulum|
|Tissue Location||Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, fetal liver and fetal heart.|
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Provided below are standard protocols that you may find useful for product applications.
Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).
Feng, T., et al. Hum. Genet. 128(3):269-280(2010)
Luo, Y., et al. J. Biol. Chem. 281(14):9385-9392(2006)
Luo, Y., et al. J. Biol. Chem. 281(14):9393-9399(2006)
Menzel, O., et al. Genomics 84(2):320-330(2004)
Martinez-Duncker, I., et al. Glycobiology 13 (12), 1C-5C (2003) :
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