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GK Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GK Antibody (N-term) AP12927A
    Western blot analysis of GK (arrow) using rabbit polyclonal GK Antibody (N-term) (Cat. #AP12927a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GK gene.
  • IHC-P - GK Antibody (N-term) AP12927A
    GK Antibody (N-term) (Cat. #AP12927a)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GK Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P32189
Other Accession Q63060, Q64516, Q0IID9, Q14409, NP_976325.1, NP_001121599.1
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 61245 Da
Antigen Region 24-51 aa
Additional Information
Gene ID 2710
Other Names Glycerol kinase, GK, Glycerokinase, ATP:glycerol 3-phosphotransferase, GK
Target/Specificity This GK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-51 amino acids from the N-terminal region of human GK.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GK
Function Key enzyme in the regulation of glycerol uptake and metabolism.
Cellular Location Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Note=In sperm and fetal tissues, the majority of the enzyme is bound to mitochondria, but in adult tissues, such as liver found in the cytoplasm
Tissue Location Highly expressed in the liver, kidney and testis. Isoform 2 and isoform 3 are expressed specifically in testis and fetal liver, but not in the adult liver
Research Areas
Citations (0)

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The product of this gene belongs to the FGGY kinase family of proteins and encodes glycerol kinase. Glycerol kinase is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Defects in this gene are the cause of glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been identified.


Lu, Y., et al. J. Lipid Res. 49(12):2582-2589(2008)
Zhang, Y.H., et al. Pediatr. Res. 59 (4 PT 1), 590-592 (2006) :
Ohira, R.H., et al. Biochem. Biophys. Res. Commun. 331(1):239-246(2005)
Stepanian, S.V., et al. Mol. Genet. Metab. 80(4):412-418(2003)
Hellerud, C., et al. Clin. Chem. Lab. Med. 41(1):46-55(2003)

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$ 295.00
$ 99.00
Cat# AP12927A
(40 western blots)
Availability: In Stock
Bulk Size
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