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EIF3A Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - EIF3A Antibody (C-term) AP12955b
    EIF3A Antibody (C-term) (Cat. #AP12955b) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the EIF3A antibody detected the EIF3A protein (arrow).
  • IHC-P - EIF3A Antibody (C-term) AP12955b
    EIF3A Antibody (C-term) (Cat. #AP12955b)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF3A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q14152
Other Accession Q1JU68, P23116, NP_003741.1
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 166569 Da
Antigen Region 1219-1247 aa
Additional Information
Gene ID 8661
Other Names Eukaryotic translation initiation factor 3 subunit A {ECO:0000255|HAMAP-Rule:MF_03000}, eIF3a {ECO:0000255|HAMAP-Rule:MF_03000}, Eukaryotic translation initiation factor 3 subunit 10 {ECO:0000255|HAMAP-Rule:MF_03000}, eIF-3-theta {ECO:0000255|HAMAP-Rule:MF_03000}, eIF3 p167, eIF3 p180, eIF3 p185, EIF3A {ECO:0000255|HAMAP-Rule:MF_03000}
Target/Specificity This EIF3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1219-1247 amino acids from the C-terminal region of human EIF3A.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF3A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF3A {ECO:0000255|HAMAP-Rule:MF_03000}
Function Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03000, ECO:0000269|PubMed:9150439}
Citations (0)

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Background

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

References

Enchev, R.I., et al. Structure 18(4):518-527(2010)
Couch, F.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(1):251-257(2010)
Dong, Z., et al. Exp. Cell Res. 315(11):1889-1894(2009)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
Martineau, Y., et al. Mol. Cell. Biol. 28(21):6658-6667(2008)

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$ 295.00
$ 99.00
Cat# AP12955b
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Quantity:
(40 western blots)
Availability: In Stock
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