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SIM1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - SIM1 Antibody (N-term) AP12960A
    Western blot analysis of lysate from 293T cell line, using SIM1 Antibody (N-term) (Cat. #AP12960a). AP12960a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
  • IHC-P - SIM1 Antibody (N-term) AP12960A
    SIM1 Antibody (N-term) (Cat. #AP12960a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SIM1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P81133
Other Accession P05709, Q61045, NP_005059.2, F1QMF7
Reactivity Human
Predicted Zebrafish, Mouse, Drosophila
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 1-30 aa
Additional Information
Other Names Single-minded homolog 1, Class E basic helix-loop-helix protein 14, bHLHe14, SIM1, BHLHE14
Target/Specificity This SIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SIM1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSIM1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SIM1
Synonyms BHLHE14
Function Transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00632, ECO:0000255|PROSITE-ProRule:PRU00981} EMBL; U70212; AAB62395.1; -; mRNA EMBL; AL121948; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; Z86062; -; NOT_ANNOTATED_CDS; Genomic_DNA CCDS; CCDS5045.1; - RefSeq; NP_005059.2; NM_005068.2 RefSeq; XP_005267157.1; XM_005267100.2 RefSeq; XP_016866686.1; XM_017011197.1 UniGene; Hs.520293; - UniGene; Hs.735754; - ProteinModelPortal; P81133; - SMR; P81133; - BioGrid; 112383; 2 IntAct; P81133; 1 STRING; 9606.ENSP00000262901; - iPTMnet; P81133; - PhosphoSitePlus; P81133; - BioMuta; SIM1; - DMDM; 109940166; - PaxDb; P81133; - PeptideAtlas; P81133; - PRIDE; P81133; - ProteomicsDB; 57691; - DNASU; 6492; - Ensembl; ENST00000262901; ENSP00000262901; ENSG00000112246 Ensembl; ENST00000369208; ENSP00000358210; ENSG00000112246 GeneID; 6492; - KEGG; hsa:6492; - UCSC; uc063qgu.1; human CTD; 6492; - DisGeNET; 6492; - EuPathDB; HostDB:ENSG00000112246.9; - GeneCards; SIM1; - HGNC; HGNC:10882; SIM1 HPA; HPA024385; - MalaCards; SIM1; - MIM; 603128; gene neXtProt; NX_P81133; - OpenTargets; ENSG00000112246; - Orphanet; 171829; 6q16 deletion syndrome Orphanet; 369873; Obesity due to SIM1 deficiency Orphanet; 398079; Prader-Willi-like syndrome due to point mutation PharmGKB; PA35782; - eggNOG; KOG3559; Eukaryota eggNOG; ENOG410XY57; LUCA GeneTree; ENSGT00760000118788; - HOGENOM; HOG000236298; - HOVERGEN; HBG003936; - InParanoid; P81133; - KO; K09100; - OMA; ISPNCFG; - OrthoDB; EOG091G06FQ; - PhylomeDB; P81133; - TreeFam; TF317772; - SIGNOR; P81133; - ChiTaRS; SIM1; human GeneWiki; SIM1; - GenomeRNAi; 6492; - PRO; PR:P81133; - Proteomes; UP000005640; Chromosome 6 Bgee; ENSG00000112246; - CleanEx; HS_SIM1; - Genevisible; P81133; HS GO; GO:0005634; C:nucleus; IBA:GO_Central GO; GO:0003677; F:DNA binding; TAS:ProtInc GO; GO:0003700; F:DNA binding transcription factor activity; TAS:ProtInc GO; GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB GO; GO:0000981; F:RNA polymerase II transcription factor activity, sequence-specific DNA binding; ISA:NTNU_SB GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW GO; GO:0007399; P:nervous system development; IBA:GO_Central GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-KW GO; GO:0001657; P:ureteric bud development; IEA:Ensembl CDD; cd00083; HLH; 1 CDD; cd00130; PAS; 2 Gene3D; 4.10.280.10; -; 1 InterPro; IPR011598; bHLH_dom InterPro; IPR036638; HLH_DNA-bd_sf InterPro; IPR001610; PAC InterPro; IPR000014; PAS InterPro; IPR035965; PAS-like_dom_sf InterPro; IPR013767; PAS_fold InterPro; IPR013655; PAS_fold_3 InterPro; IPR010578; SIM_C Pfam; PF00010; HLH; 1 Pfam; PF00989; PAS; 1 Pfam; PF08447; PAS_3; 1 Pfam; PF06621; SIM_C; 1 SMART; SM00353; HLH; 1 SMART; SM00086; PAC; 1 SMART; SM00091; PAS; 2 SUPFAM; SSF47459; SSF47459; 1 SUPFAM; SSF55785; SSF55785; 2 PROSITE; PS50888; BHLH; 1 PROSITE; PS50112; PAS; 2 PROSITE; PS51302; SIM_C; 1 2: Evidence at transcript level; Complete proteome; Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation CHAIN 1 766 Single-minded homolog 1 /FTId=PRO_0000127439 DOMAIN 1 53 bHLH. {ECO:0000255|PROSITE- ProRule:PRU00981} DOMAIN 77 147 PAS 1. {ECO:0000255|PROSITE- ProRule:PRU00140} DOMAIN 218 288 PAS 2. {ECO:0000255|PROSITE- ProRule:PRU00140} DOMAIN 292 335 PAC DOMAIN 336 766 Single-minded C-terminal {ECO:0000255|PROSITE-ProRule:PRU00632} MOTIF 368 387 Nuclear localization signal VARIANT 175 175 L -> F (in dbSNP:rs438766) /FTId=VAR_049549 VARIANT 352 352 P -> T (in dbSNP:rs3734354) /FTId=VAR_034496 VARIANT 371 371 A -> V (in dbSNP:rs3734355) /FTId=VAR_034497 CONFLICT 30 30 P -> A (in Ref. 1; AAB62395) CONFLICT 37 37 L -> V (in Ref. 1; AAB62395) SEQUENCE 766 AA; 85515 MW; 05988D428A84431F CRC64; MKEKSKNAAR TRREKENSEF YELAKLLPLP SAITSQLDKA SIIRLTTSYL KMRVVFPEGL GEAWGHSSRT SPLDNVGREL GSHLLQTLDG FIFVVAPDGK IMYISETASV HLGLSQVELT GNSIYEYIHP ADHDEMTAVL TAHQPYHSHF VQEYEIERSF FLRMKCVLAK RNAGLTCGGY KVIHCSGYLK IRQYSLDMSP FDGCYQNVGL VAVGHSLPPS AVTEIKLHSN MFMFRASLDM KLIFLDSRVA ELTGYEPQDL IEKTLYHHVH GCDTFHLRCA HHLLLVKGQV TTKYYRFLAK HGGWVWVQSY ATIVHNSRSS RPHCIVSVNY VLTDTEYKGL QLSLDQISAS KPAFSYTSSS TPTMTDNRKG AKSRLSSSKS KSRTSPYPQY SGFHTERSES DHDSQWGGSP LTDTASPQLL DPADRPGSQH DASCAYRQFS DRSSLCYGFA LDHSRLVEER HFHTQACEGG RCEAGRYFLG TPQAGREPWW GSRAALPLTK ASPESREAYE NSMPHIASVH RIHGRGHWDE DSVVSSPDPG SASESGDRYR TEQYQSSPHE PSKIETLIRA TQQMIKEEEN RLQLRKAPSD QLASINGAGK KHSLCFANYQ QPPPTGEVCH GSALANTSPC DHIQQREGKM LSPHENDYDN SPTALSRISS PNSDRISKSS LILAKDYLHS DISPHQTAGD HPTVSPNCFG SHRQYFDKHA YTLTGYALEH LYDSETIRNY SLGCNGSHFD VTSHLRMQPD PAQGHKGTSV IITNGS
Research Areas
Citations (0)

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Background

SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or mental retardation of Down syndrome.

References

Ghoussaini, M., et al. Obesity (Silver Spring) 18(8):1670-1675(2010)
Tolson, K.P., et al. J. Neurosci. 30(10):3803-3812(2010)
Traurig, M., et al. Diabetes 58(7):1682-1689(2009)
Gregorio, S.P., et al. Psychiatry Res 165 (1-2), 1-9 (2009) :
Hung, C.C., et al. Int J Obes (Lond) 31(3):429-434(2007)

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Cat# AP12960A
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