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SFRP5 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SFRP5 Antibody (Center) AP12966C
    Anti-SFRP5 Antibody (Center) at 1:2000 dilution + human pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - SFRP5 Antibody (Center) AP12966C
    SFRP5 Antibody (Center) (Cat. #AP12966c) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the SFRP5 antibody detected the SFRP5 protein (arrow).
  • IHC-P - SFRP5 Antibody (Center) AP12966C
    SFRP5 Antibody (Center) (Cat. #AP12966c)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SFRP5 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q5T4F7
Other Accession Q9XSC1, NP_003006.2
Reactivity Human, Mouse
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35563 Da
Antigen Region 190-219 aa
Additional Information
Gene ID 6425
Other Names Secreted frizzled-related protein 5, sFRP-5, Frizzled-related protein 1b, FRP-1b, Secreted apoptosis-related protein 3, SARP-3, SFRP5, FRP1B, SARP3
Target/Specificity This SFRP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-219 amino acids from the Central region of human SFRP5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSFRP5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SFRP5
Synonyms FRP1B, SARP3
Function Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina.
Cellular Location Secreted.
Tissue Location Highly expressed in the retinal pigment epithelium (RPE) and pancreas. Weak expression in heart, liver and muscle.
Research Areas
Citations (0)

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Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq].


Su, H.Y., et al. Int. J. Cancer 127(3):555-567(2010)
Sharma, S., et al. Am. J. Respir. Crit. Care Med. 181(4):328-336(2010)
Jost, E., et al. Cancer Lett. 281(1):24-31(2009)
Zhao, C., et al. BMC Cancer 9, 224 (2009) :
Tanaka, J., et al. Hepatogastroenterology 55(85):1265-1268(2008)

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Cat# AP12966C
(40 western blots)
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