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GIF Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - GIF Antibody (Center) AP12975C
    GIF Antibody (Center) (Cat. #AP12975c) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GIF antibody detected the GIF protein (arrow).
  • IHC-P - GIF Antibody (Center) AP12975C
    GIF Antibody (Center) (Cat. #AP12975c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GIF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IF - GIF Antibody (Center) AP12975C
    Confocal immunofluorescent analysis of GIF Antibody (Center) (Cat#AP12975c) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • FC - GIF Antibody (Center) AP12975C
    GIF Antibody (Center) (Cat. #AP12975c) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P27352
Other Accession NP_005133.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 45416 Da
Antigen Region 189-218 aa
Additional Information
Gene ID 2694
Other Names Gastric intrinsic factor, Intrinsic factor, IF, INF, GIF, IFMH
Target/Specificity This GIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the Central region of human GIF.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGIF Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GIF
Synonyms IFMH
Function Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis.
Cellular Location Secreted.
Tissue Location Gastric mucosa.
Research Areas
Citations (0)

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This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia.


Levine, A.J., et al. Cancer Epidemiol. Biomarkers Prev. 19(7):1812-1821(2010)
Andersen, C.B., et al. Nature 464(7287):445-448(2010)
Ament, A.E., et al. Br. J. Haematol. 144(4):622-624(2009)
Remacha, A.F., et al. Ann. Hematol. 87(7):599-600(2008)
Mathews, F.S., et al. Proc. Natl. Acad. Sci. U.S.A. 104(44):17311-17316(2007)

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$ 295.00
$ 99.00
Cat# AP12975C
(40 western blots)
Availability: In Stock
Bulk Size
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