|Application ||WB, E|
|Calculated MW||107383 Da|
|Antigen Region||914-943 aa|
|Other Names||Chromosome transmission fidelity protein 18 homolog, hCTF18, CHL12, CHTF18, C16orf41, CTF18|
|Target/Specificity||This CHTF18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 914-943 amino acids from the C-terminal region of human CHTF18.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CHTF18 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Chromosome cohesion factor involved in sister chromatid cohesion and fidelity of chromosome transmission. Component of one of the cell nuclear antigen loader complexes, CTF18-replication factor C (CTF18-RFC), which consists of CTF18, CTF8, DCC1, RFC2, RFC3, RFC4 and RFC5. The CTF18-RFC complex binds to single- stranded and primed DNAs and has weak ATPase activity that is stimulated by the presence of primed DNA, replication protein A (RPA) and by proliferating cell nuclear antigen (PCNA). The CTF18- RFC complex catalyzes the ATP-dependent loading of PCNA onto primed and gapped DNA. It also interacts with and stimulates DNA polymerase POLH.|
|Cellular Location||Nucleus. Note=Associates with chromatin during S phase|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
CHTF18, CHTF8 (MIM 613202), and DCC1 (DSCC1; MIM 613203) are components of an alternative replication factor C (RFC) (see MIM 600404) complex that loads PCNA (MIM 176740) onto DNA during S phase of the cell cycle (Merkle et al., 2003 [PubMed 12766176]; Bermudez et al., 2003 [PubMed 12930902]).
Murakami, T., et al. J. Biol. Chem. 285(45):34608-34615(2010)
Farina, A., et al. J. Biol. Chem. 283(30):20925-20936(2008)
Shiomi, Y., et al. J. Biol. Chem. 282(29):20906-20914(2007)
Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
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