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GST Tag Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GST Tag Antibody AP1298a
    The anti-GST Pab (Cat. #AP1298a) is used in Western blot to detect a GST-fusion recombinant protein (42 kDa) purified from bacterial lysate (Lanes 1-3: 10, 40, and 160 ng GST-fusion protein).
  • SPECIFICATION
  • CITATIONS: 3
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional Information
Other Names Green Fluorescent Protein
Target/Specificity This GST Tag antibody is generated from rabbits immunized with a recombinant full length of GST.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGST Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas
Citations ( 0 )

Background

Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using the Abgent anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.

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$ 295.00
$ 99.00
Cat# AP1298a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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