|Application ||WB, E|
|Other Accession||NP_001159367.1, NP_057663.1|
|Calculated MW||28991 Da|
|Antigen Region||153-182 aa|
|Other Names||CD320 antigen, 8D6 antigen, FDC-signaling molecule 8D6, FDC-SM-8D6, Transcobalamin receptor, TCblR, CD320, CD320, 8D6A|
|Target/Specificity||This CD320 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-182 amino acids from the C-terminal region of human CD320.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD320 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for transcobalamin saturated with cobalamin (TCbl) (PubMed:18779389). Plays an important role in cobalamin uptake (PubMed:18779389, PubMed:20524213). Plasma membrane protein that is expressed on follicular dendritic cells (FDC) and mediates interaction with germinal center B cells (PubMed:10727470). Functions as costimulator to promote B cell responses to antigenic stimuli; promotes B cell differentiation and proliferation (PubMed:10727470, PubMed:11418631). Germinal center-B (GC-B) cells differentiate into memory B-cells and plasma cells (PC) through interaction with T-cells and follicular dendritic cells (FDC) (PubMed:11418631). CD320 augments the proliferation of PC precursors generated by IL-10 (PubMed:11418631).|
|Cellular Location||Cell membrane; Single- pass type I membrane protein|
|Tissue Location||Detected in the germinal center (GC) of lymphoid follicles (at protein level) (PubMed:11418631). Expressed abundantly on follicular dendritic cells (FDCs) (PubMed:10727470)|
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Provided below are standard protocols that you may find useful for product applications.
The CD320 antigen gene encodes the transcobalamin receptor (TCBLR). Cellular uptake of cobalamin (vitamin B12) is mediated by receptors expressed on the cell surface. Transcobalamin II (TCN2; MIM 613441), a plasma protein secreted by endothelial cells, binds the cobalamin absorbed in the distal ileum and carries 10 to 30% of total circulating cobalamin. CD320 encodes a transcobalamin receptor that binds TCN2-cobalamin at the plasma membrane and internalizes the complex by endocytosis (Quadros et al., 2009 [PubMed 18779389]).
Jiang, W., et al. Gene 466 (1-2), 49-55 (2010) :
Pangilinan, F., et al. J. Med. Genet. 47(10):677-685(2010)
Quadros, E.V., et al. Hum. Mutat. 31(8):924-929(2010)
Quadros, E.V., et al. Blood 113(1):186-192(2009)
Cho, W., et al. BMB Rep 41(12):863-867(2008)
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