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PRLH Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PRLH Antibody (Center) AP13150c
    PRLH Antibody (Center) (Cat. #AP13150c) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the PRLH antibody detected the PRLH protein (arrow).
  • IHC-P - PRLH Antibody (Center) AP13150c
    PRLH Antibody (Center) (Cat. #AP13150c)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PRLH Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P81277
Other Accession NP_056977.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 27-56 aa
Additional Information
Other Names Prolactin-releasing peptide, PrRP, Prolactin-releasing hormone, Prolactin-releasing peptide PrRP31, Prolactin-releasing peptide PrRP20, PRLH, PRH
Target/Specificity This PRLH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the Central region of human PRLH.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRLH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRLH
Synonyms PRH
Function Stimulates prolactin (PRL) release and regulates the expression of prolactin through its receptor GPR10. May stimulate lactotrophs directly to secrete PRL.
Cellular Location Secreted.
Tissue Location Medulla oblongata and hypothalamus. EMBL; AB015419; BAA29027.1; -; mRNA EMBL; BC069081; AAH69081.1; -; mRNA EMBL; BC069284; AAH69284.1; -; mRNA CCDS; CCDS2519.1; - RefSeq; NP_056977.1; NM_015893.1 UniGene; Hs.247710; - ProteinModelPortal; P81277; - STRING; 9606.ENSP00000165524; - iPTMnet; P81277; - PhosphoSitePlus; P81277; - BioMuta; PRLH; - DMDM; 8134649; - PaxDb; P81277; - PRIDE; P81277; - Ensembl; ENST00000165524; ENSP00000165524; ENSG00000071677 GeneID; 51052; - KEGG; hsa:51052; - CTD; 51052; - DisGeNET; 51052; - GeneCards; PRLH; - HGNC; HGNC:17945; PRLH HPA; HPA014768; - MIM; 602663; gene neXtProt; NX_P81277; - OpenTargets; ENSG00000071677; - PharmGKB; PA134927756; - eggNOG; ENOG410J49T; Eukaryota eggNOG; ENOG4111800; LUCA GeneTree; ENSGT00390000005489; - HOGENOM; HOG000115720; - HOVERGEN; HBG004601; - InParanoid; P81277; - KO; K05269; - OMA; INPAWYA; - OrthoDB; EOG091G191K; - PhylomeDB; P81277; - TreeFam; TF330717; - BioCyc; ZFISH:ENSG00000071677-MONOMER; - Reactome; R-HSA-375276; Peptide ligand-binding receptors GeneWiki; PRLH; - GeneWiki; Prolactin-releasing_peptide; - GenomeRNAi; 51052; - PRO; PR:P81277; - Proteomes; UP000005640; Chromosome 2 Bgee; ENSG00000071677; - CleanEx; HS_PRLH; - ExpressionAtlas; P81277; baseline and differential Genevisible; P81277; HS GO; GO:0005737; C:cytoplasm; IEA:Ensembl GO; GO:0005576; C:extracellular region; TAS:Reactome GO; GO:0005179; F:hormone activity; TAS:ProtInc GO; GO:0048483; P:autonomic nervous system development; IEA:Ensembl GO; GO:0006112; P:energy reserve metabolic process; IEA:Ensembl GO; GO:0045444; P:fat cell differentiation; IEA:Ensembl GO; GO:0007186; P:G-protein coupled receptor signaling pathway; IEA:Ensembl GO; GO:0006629; P:lipid metabolic process; IEA:Ensembl GO; GO:0002023; P:reduction of food intake in response to dietary excess; IEA:Ensembl GO; GO:0040014; P:regulation of multicellular organism growth; IEA:Ensembl GO; GO:0009749; P:response to glucose; IEA:Ensembl GO; GO:0032868; P:response to insulin; IEA:Ensembl GO; GO:0001894; P:tissue homeostasis; IEA:Ensembl InterPro; IPR026194; PrRP PANTHER; PTHR17206; PTHR17206; 1 Pfam; PF15172; Prolactin_RP; 1 2: Evidence at transcript level; Amidation; Cleavage on pair of basic residues; Complete proteome; Hormone; Reference proteome; Secreted; Signal SIGNAL 1 22 PEPTIDE 23 53 Prolactin-releasing peptide PrRP31 /FTId=PRO_0000022144 PEPTIDE 34 53 Prolactin-releasing peptide PrRP20 /FTId=PRO_0000022145 PROPEP 58 87 /FTId=PRO_0000022146 MOD_RES 53 53 Phenylalanine amide. SEQUENCE 87 AA; 9639 MW; 229A2F3F50CF981B CRC64; MKVLRAWLLC LLMLGLALRG AASRTHRHSM EIRTPDINPA WYASRGIRPV GRFGRRRATL GDVPKPGLRP RLTCFPLEGG AMSSQDG
Citations (0)

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Background

Stimulates prolactin (PRL) release and regulates the expression of prolactin through its receptor GPR10. May stimulate lactotrophs directly to secrete PRL.

References

Lagerstrom, M.C., et al. Ann. N. Y. Acad. Sci. 1040, 368-370 (2005) :
Takahashi, K., et al. Peptides 23(6):1135-1140(2002)
Yasui, Y., et al. Endocr. J. 48(3):397-401(2001)
Langmead, C.J., et al. Br. J. Pharmacol. 131(4):683-688(2000)
Zhang, X., et al. J. Clin. Endocrinol. Metab. 84(12):4652-4655(1999)

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$ 295.00
$ 99.00
Cat# AP13150c
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(40 western blots)
Availability: In Stock
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