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EIF1AY Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - EIF1AY Antibody (N-term) AP13157a
    EIF1AY Antibody (N-term) (Cat. #AP13157a) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the EIF1AY antibody detected the EIF1AY protein (arrow).
  • IHC-P - EIF1AY Antibody (N-term) AP13157a
    EIF1AY Antibody (N-term) (Cat. #AP13157a)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF1AY Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession O14602
Other Accession Q6VV72, P47814, Q60872, Q8BMJ3, P47813, NP_004672.2
Reactivity Human
Predicted Mouse, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 16442 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 9086
Other Names Eukaryotic translation initiation factor 1A, Y-chromosomal, eIF-1A Y isoform, Eukaryotic translation initiation factor 4C, eIF-4C, EIF1AY
Target/Specificity This EIF1AY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EIF1AY.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEIF1AY Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name EIF1AY
Function Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits (By similarity).
Tissue Location Ubiquitous.
Citations (0)

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Background

This gene encodes a protein similar to eukaryotic translation initiation factor 1A (EIF1A). EIF1A is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA.

References

Serajee, F.J., et al. J. Child Neurol. 24(10):1258-1261(2009)
Lim, J., et al. Cell 125(4):801-814(2006)
Fortna, A., et al. PLoS Biol. 2 (7), E207 (2004) :
Agate, R.J., et al. Mol. Biol. Evol. 21(2):384-396(2004)
Skaletsky, H., et al. Nature 423(6942):825-837(2003)

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$ 295.00
$ 99.00
Cat# AP13157a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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