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CAPN8 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CAPN8 Antibody (Center) AP13161c
    CAPN8 Antibody (Center) (Cat. #AP13161c) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CAPN8 antibody detected the CAPN8 protein (arrow).
  • IHC-P - CAPN8 Antibody (Center) AP13161c
    CAPN8 Antibody (Center) (Cat. #AP13161c)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAPN8 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession A6NHC0
Other Accession NP_001137434.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 79144 Da
Antigen Region 290-318 aa
Additional Information
Gene ID 388743
Other Names Calpain-8, New calpain 2, nCL-2, Stomach-specific M-type calpain, CAPN8, NCL2
Target/Specificity This CAPN8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-318 amino acids from the Central region of human CAPN8.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCAPN8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CAPN8
Synonyms NCL2
Function Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta- subunit of coatomer complex (By similarity).
Cellular Location Cytoplasm. Golgi apparatus
Tissue Location Stomach.
Research Areas
Citations (0)

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Background

Calcium-regulated non-lysosomal thiol-protease. Involved in membrane trafficking in the gastric surface mucus cells (pit cells) and may involve the membrane trafficking of mucus cells via interactions with coat protein. Proteolytically cleaves the beta-subunit of coatomer complex (By similarity).

References

Hata, S., et al. J. Biol. Chem. 282(38):27847-27856(2007)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Hata, S., et al. J. Mol. Evol. 53(3):191-203(2001)
Huang, Y., et al. Trends Mol Med 7(8):355-362(2001)
Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001)

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$ 295.00
$ 99.00
Cat# AP13161c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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