|Application ||WB, IHC-P, E|
|Calculated MW||85722 Da|
|Antigen Region||543-572 aa|
|Other Names||E3 ubiquitin-protein ligase RNF43, 632-, RING finger protein 43, RNF43|
|Target/Specificity||This RNF43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543-572 amino acids from the C-terminal region of human RNF43.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RNF43 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus envelope. Note=According to a report, may be secreted.|
|Tissue Location||Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines|
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Provided below are standard protocols that you may find useful for product applications.
RNF43 is a HAP95 (AKAP8L; MIM 609475) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer (Yagyu et al., 2004 [PubMed 15492824]; Sugiura et al., 2008 [PubMed 18313049]).
Low, S.K., et al. PLoS ONE 5 (7), E11824 (2010) :
Sugiura, T., et al. Exp. Cell Res. 314(7):1519-1528(2008)
Uchida, N., et al. Clin. Cancer Res. 10(24):8577-8586(2004)
Yagyu, R., et al. Int. J. Oncol. 25(5):1343-1348(2004)
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