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RNF43 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP13204b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP13204b-ev20 | Ev20 size 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
RNF43 Antibody (C-term) - Product info | |
| Application | WB, IHC
|
| Primary Accession | Q68DV7 |
| Other Accession | NP_060233.3 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 85722 Da |
RNF43 Antibody (C-term) - Additional info | |
| Gene ID 54894 | |
| Other Names RNF43; E3 ubiquitin-protein ligase RNF43; RING finger protein 43 | |
| Target/Specificity This RNF43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 542-572 amino acids from the C-terminal region of human RNF43. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions RNF43 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
RNF43 Antibody (C-term) - Protein Information | |
| Name RNF43 | |
| Function E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone | |
| Cellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus envelope. Note=According to a report, may be secreted (PubMed:15492824) | |
| Tissue Location Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines | |
RNF43 Antibody (C-term) - Related products
RNF43 Antibody (C-term) - Application data
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RNF43 Antibody (C-term) (Cat. #AP13204b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RNF43 antibody detected the RNF43 protein (arrow).
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RNF43 Antibody (C-term) (Cat. #AP13204b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF43 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
RNF43 Antibody (C-term) - Research Areas
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BACKGROUND
RNF43 is a HAP95 (AKAP8L; MIM 609475) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer (Yagyu et al., 2004 [PubMed 15492824]; Sugiura et al., 2008 [PubMed 18313049]).
REFERENCES
Low, S.K., et al. PLoS ONE 5 (7), E11824 (2010) : Sugiura, T., et al. Exp. Cell Res. 314(7):1519-1528(2008) Uchida, N., et al. Clin. Cancer Res. 10(24):8577-8586(2004) Yagyu, R., et al. Int. J. Oncol. 25(5):1343-1348(2004)
