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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   RNF43 Antibody (C-term)   

RNF43 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - RNF43 Antibody (C-term) AP13204B
    RNF43 Antibody (C-term) (Cat. #AP13204b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the RNF43 antibody detected the RNF43 protein (arrow).
  • IHC-P - RNF43 Antibody (C-term) AP13204B
    RNF43 Antibody (C-term) (Cat. #AP13204b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RNF43 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q68DV7
Other Accession NP_060233.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 85722 Da
Antigen Region 543-572 aa
Additional Information
Gene ID 54894
Other Names E3 ubiquitin-protein ligase RNF43, 632-, RING finger protein 43, RNF43
Target/Specificity This RNF43 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 543-572 amino acids from the C-terminal region of human RNF43.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRNF43 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RNF43
Function E3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Acts as a tumor suppressor in the intestinal stem cell zone by inhibiting the Wnt signaling pathway, thereby resticting the size of the intestinal stem cell zone.
Cellular Location Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Nucleus envelope. Note=According to a report, may be secreted.
Tissue Location Expressed in fetal kidney, fetal lung, in colon cancer tissues, hepatocellular carcinomas and lung adenocarcinomas. Overexpressed in colorectal cancer cell lines
Research Areas
Citations (0)

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Background

RNF43 is a HAP95 (AKAP8L; MIM 609475) binding ubiquitin ligase that promotes cell growth and is upregulated in colon cancer (Yagyu et al., 2004 [PubMed 15492824]; Sugiura et al., 2008 [PubMed 18313049]).

References

Low, S.K., et al. PLoS ONE 5 (7), E11824 (2010) :
Sugiura, T., et al. Exp. Cell Res. 314(7):1519-1528(2008)
Uchida, N., et al. Clin. Cancer Res. 10(24):8577-8586(2004)
Yagyu, R., et al. Int. J. Oncol. 25(5):1343-1348(2004)

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Discontinued
Cat# AP13204B
Size:
(40 western blots)

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