|Application ||WB, E|
|Calculated MW||116159 Da|
|Antigen Region||116-145 aa|
|Other Names||NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein, Clan protein, Caspase recruitment domain-containing protein 12, Ice protease-activating factor, Ipaf, NLRC4, CARD12, CLAN, CLAN1, IPAF|
|Target/Specificity||This NLRC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-145 amino acids from the N-terminal region of human NLRC4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NLRC4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||CARD12, CLAN, CLAN1, IPAF|
|Function||Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis.|
|Cellular Location||Cytoplasm. Cytoplasm, cytosol. Note=Cytoplasmic filaments|
|Tissue Location||Isoform 2 is expressed ubiquitously, although highly expressed in lung and spleen. Isoform 1 is highly expressed in lung, followed by leukocytes especially monocytes, lymph node, colon, brain, prostate, placenta, spleen, bone marrow and fetal liver. Isoform 4 is only detected in brain|
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Provided below are standard protocols that you may find useful for product applications.
In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4 (MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.
Miao, E.A., et al. J. Clin. Immunol. 30(4):502-506(2010)
Vinzing, M., et al. J. Immunol. 180(10):6808-6815(2008)
Macaluso, F., et al. Exp. Dermatol. 16(8):692-698(2007)
Bruey, J.M., et al. Cell 129(1):45-56(2007)
Thalappilly, S., et al. FEBS J. 273(12):2766-2778(2006)
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