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WIPI2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - WIPI2 Antibody (N-term) AP13314a
    WIPI2 Antibody (N-term) (Cat. #AP13314a) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the WIPI2 antibody detected the WIPI2 protein (arrow).
  • IHC-P - WIPI2 Antibody (N-term) AP13314a
    WIPI2 Antibody (N-term) (Cat. #AP13314a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WIPI2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y4P8
Other Accession NP_001028690.1, NP_057087.2, NP_001028691.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49408 Da
Antigen Region 4-32 aa
Additional Information
Gene ID 26100
Other Names WD repeat domain phosphoinositide-interacting protein 2, WIPI-2, WIPI49-like protein 2, WIPI2
Target/Specificity This WIPI2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human WIPI2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsWIPI2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name WIPI2
Function Early component of the autophagy machinery being involved in formation of preautophagosomal structures and their maturation into mature phagosomes in response to phosphatidylinositol 3-phosphate (PtdIns3P). May bind PtdIns3P.
Cellular Location Preautophagosomal structure membrane; Peripheral membrane protein; Cytoplasmic side Note=Localizes to omegasomes membranes which are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P.
Tissue Location Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas Expression is down-regulated in pancreatic and in kidney tumors
Research Areas
Citations ( 0 )


WD40 repeat proteins are key components of many essential biologic functions. They regulate the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids (Proikas-Cezanne et al., 2004 [PubMed 15602573]).


Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Proikas-Cezanne, T., et al. Oncogene 23(58):9314-9325(2004)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)

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$ 295.00
$ 99.00
Cat# AP13314a
(40 western blots)
Availability: In Stock
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