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HSP40 AntibodyPurified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1334a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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HSP40 Antibody - Product info | |
| Application | WB, IHC
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| Primary Accession | P25685 |
| Other Accession | NP_006136 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 38044 Da |
HSP40 Antibody - Additional info | |
| Gene ID 3337 | |
| Other Names DNAJB1; DNAJ1; HDJ1; HSPF1; DnaJ homolog subfamily B member 1; DnaJ protein homolog 1; Heat shock 40 kDa protein 1; Human DnaJ protein 1 | |
| Target/Specificity This HSP40 antibody is generated from rabbits immunized with a recombinant protein encoding full length of human HSP40. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions HSP40 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
HSP40 Antibody - Protein Information | |
| Name DNAJB1 | |
| Synonyms DNAJ1, HDJ1, HSPF1 | |
| Function Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP | |
| Cellular Location Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Translocates rapidly from the cytoplasm to the nucleus, and especially to the nucleoli, upon heat shock | |
HSP40 Antibody - Related products
HSP40 Antibody - Application data
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Western blot analysis of anti-HSP40 Antibody (Cat.#AP1334a) in Hela cell line lysates (35ug/lane). HSP40 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with HSP40 Antibody (Cat.#AP1334a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HSP40 Antibody - Research Areas
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BACKGROUND
DnaJ (Hsp40) belongs to the DnaJ-class of molecular chaperones with a C-terminal Zn finger domain. HSP40 (DnaJ) together with DnaK and GrpE form a molecular chaperone that is involved in formation of protein complexes, protein folding, prevention of protein aggregation, and protein turnover and export. Several human neurodegenerative diseases involve the expansion of a polyglutamine within the disease proteins. Molecular chaperones such as HSP40 complexes can modulate polyglutamine pathogenesis In transgenic Drosophila disease models of Machado-Joseph disease and Huntington disease Hdj1, the Drosophila homolog to human HSP40, demonstrates substrate specificity for polyglutamine proteins suppression in combination with other molecular chapterones of neurotoxicity, and altered solubility of mutant polyglutamine proteins.
REFERENCES
Ohtsuka, K., et al., Cell Stress Chaperones 5(2):98-112 (2000). Hata, M., et al., Biochim. Biophys. Acta 1397(1):43-55 (1998). Hata, M., et al., Genomics 38(3):446-449 (1996). Ohtsuka, K., Biochem. Biophys. Res. Commun. 197(1):235-240 (1993).
