|Application ||WB, E|
|Other Accession||NP_065166.2, NP_787109.2|
|Calculated MW||74222 Da|
|Antigen Region||614-643 aa|
|Other Names||Junctophilin-2, JP-2, Junctophilin type 2, JPH2, JP2|
|Target/Specificity||This JPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 614-643 amino acids from the C-terminal region of human JPH2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||JPH2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH2 is necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release. Contributes to the construction of skeletal muscle triad junctions.|
|Cellular Location||Cell membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein Sarcoplasmic reticulum membrane; Single-pass type IV membrane protein. Note=Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic/sarcoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane. In heart cells, it predominantly associates along Z lines within myocytes. In skeletal muscle, it is specifically localized at the junction of A and I bands (By similarity).|
|Tissue Location||Specifically expressed in skeletal muscle and heart.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. This gene is a member of the junctophilin gene family. Alternative splicing has been observed at this locus and two variants encoding distinct isoforms are described.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Woo, J.S., et al. Biochem. J. 427(1):125-134(2010)
Yamazaki, D., et al. Pharmacol. Ther. 121(3):265-272(2009)
Landstrom, A.P., et al. J. Mol. Cell. Cardiol. 42(6):1026-1035(2007)
Matsushita, Y., et al. J. Hum. Genet. 52(6):543-548(2007)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.