|Application ||WB, E|
|Other Accession||NP_065166.2, NP_787109.2|
|Calculated MW||74222 Da|
|Antigen Region||614-643 aa|
|Other Names||Junctophilin-2, JP-2, Junctophilin type 2, JPH2, JP2|
|Target/Specificity||This JPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 614-643 amino acids from the C-terminal region of human JPH2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||JPH2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH2 is necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release. Contributes to the construction of skeletal muscle triad junctions.|
|Cellular Location||Cell membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein Sarcoplasmic reticulum membrane; Single-pass type IV membrane protein. Note=Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic/sarcoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane. In heart cells, it predominantly associates along Z lines within myocytes. In skeletal muscle, it is specifically localized at the junction of A and I bands (By similarity).|
|Tissue Location||Specifically expressed in skeletal muscle and heart.|
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Provided below are standard protocols that you may find useful for product applications.
Junctional complexes between the plasma membrane and endoplasmic/sarcoplasmic reticulum are a common feature of all excitable cell types and mediate cross talk between cell surface and intracellular ion channels. The protein encoded by this gene is a component of junctional complexes and is composed of a C-terminal hydrophobic segment spanning the endoplasmic/sarcoplasmic reticulum membrane and a remaining cytoplasmic domain that shows specific affinity for the plasma membrane. This gene is a member of the junctophilin gene family. Alternative splicing has been observed at this locus and two variants encoding distinct isoforms are described.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Woo, J.S., et al. Biochem. J. 427(1):125-134(2010)
Yamazaki, D., et al. Pharmacol. Ther. 121(3):265-272(2009)
Landstrom, A.P., et al. J. Mol. Cell. Cardiol. 42(6):1026-1035(2007)
Matsushita, Y., et al. J. Hum. Genet. 52(6):543-548(2007)
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