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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   MID1 Antibody (C-term)   

MID1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - MID1 Antibody (C-term) AP13465b
    MID1 Antibody (C-term) (Cat. #AP13465b) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the MID1 antibody detected the MID1 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O15344
Other Accession NP_001092094.1, NP_001180206.1, NP_000372.1, NP_001180207.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 75251 Da
Antigen Region 575-603 aa
Additional Information
Gene ID 4281
Other Names E3 ubiquitin-protein ligase Midline-1, 632-, Midin, Putative transcription factor XPRF, RING finger protein 59, RING finger protein Midline-1, Tripartite motif-containing protein 18, MID1, FXY, RNF59, TRIM18, XPRF
Target/Specificity This MID1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 575-603 amino acids from the C-terminal region of human MID1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMID1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MID1
Synonyms FXY, RNF59, TRIM18, XPRF
Function Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle Note=Microtubule-associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis
Tissue Location In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain
Research Areas
Citations (0)

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The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also known as the 'RING-B box-coiled coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein forms homodimers which associate with microtubules in the cytoplasm. The protein is likely involved in the formation of multiprotein structures acting as anchor points to microtubules. Mutations in this gene have been associated with the X-linked form of Opitz syndrome, which is characterized by midline abnormalities such as cleft lip, laryngeal cleft, heart defects, hypospadias, and agenesis of the corpus callosum. This gene was also the first example of a gene subject to X inactivation in human while escaping it in mouse. Multiple different transcript variants are generated by alternate splicing; however, the full-length nature of some of the variants has not been determined.


Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009)
Treutlein, J., et al. Arch. Gen. Psychiatry 66(7):773-784(2009)
Styrkarsdottir, U., et al. Nat. Genet. 41(1):15-17(2009)
Scapoli, L., et al. Eur. J. Oral Sci. 116(6):507-511(2008)
Aranda-Orgilles, B., et al. PLoS ONE 3 (10), E3507 (2008) :

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$ 295.00
$ 99.00
Cat# AP13465b
(40 western blots)
Availability: In Stock
Bulk Size
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