|Application ||WB, FC, E|
|Other Accession||P83871, P83870, NP_116147.1|
|Calculated MW||12405 Da|
|Antigen Region||81-110 aa|
|Other Names||PHD finger-like domain-containing protein 5A, PHD finger-like domain protein 5A, Splicing factor 3B-associated 14 kDa protein, SF3b14b, PHF5A|
|Target/Specificity||This PHF5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the C-terminal region of human PHF5A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PHF5A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a transcriptional regulator by binding to the GJA1/Cx43 promoter and enhancing its up-regulation by ESR1/ER- alpha. Also involved in pre-mRNA splicing.|
|Cellular Location||Nucleus. Nucleus speckle|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes.
Kuwasako, K., et al. Proteins 71(4):1617-1636(2008)
Will, C.L., et al. RNA 10(6):929-941(2004)
Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004) :
Will, C.L., et al. EMBO J. 21(18):4978-4988(2002)
Zhou, Z., et al. Nature 419(6903):182-185(2002)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.