|Application ||WB, E|
|Other Accession||NP_005123.2, NP_001041670.1|
|Calculated MW||62614 Da|
|Antigen Region||194-222 aa|
|Other Names||Meiotic recombination protein REC8 homolog, Cohesin Rec8p, REC8, REC8L1|
|Target/Specificity||This REC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-222 amino acids from the Central region of human REC8.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||REC8 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).|
|Cellular Location||Nucleus. Chromosome. Note=In meiotic chromosomes, localized along axial elements in prophase from the leptotene to diplotene stages. At later prophase stages, diakinesis and metaphase I, localized along interstitial axes of chromosomes including both centromere and arm regions. No longer detected in arm regions in anaphase I but persists on centromere regions until metaphase II (By similarity) Localised to centromeres and spindle poles in endopolyploid tumor cells.|
|Tissue Location||Expressed in testis and thymus. Expressed in the B-cell lines WI-L2-NS and Namalwa (at protein level)|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
Aston, K.I., et al. Hum. Reprod. 25(6):1383-1397(2010)
Erenpreisa, J., et al. Exp. Cell Res. 315(15):2593-2603(2009)
Griffin, J., et al. Syst Biol Reprod Med 54(3):163-165(2008)
Xu, H., et al. Dev. Cell 8(6):949-961(2005)
Prieto, I., et al. Chromosome Res. 12(3):197-213(2004)
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