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DHX32 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DHX32 Antibody (Center) AP13575c
    DHX32 Antibody (Center) (Cat. #AP13575c) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the DHX32 antibody detected the DHX32 protein (arrow).
    detail
  • IHC-P - DHX32 Antibody (Center) AP13575c
    DHX32 Antibody (Center) (Cat. #AP13575c)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DHX32 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q7L7V1
Other Accession NP_060650.2
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 84419 Da
Antigen Region 342-371 aa
Additional Information
Gene ID 55760
Other Names Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX32, DEAD/H box 32, DEAD/H helicase-like protein 1, DHLP1, DEAH box protein 32, HuDDX32, DHX32, DDX32
Target/Specificity This DHX32 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 342-371 amino acids from the Central region of human DHX32.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDHX32 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DHX32
Synonyms DDX32
Cellular Location Nucleus. Mitochondrion
Tissue Location Expressed in lymphoid tissues (at protein level). Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung, lymphoid tissues and blood leukocytes.
Research Areas
Citations (0)
citation

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Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a member of this family. The function of this member has not been determined. Alternative splicing of this gene generates 2 transcript variants, but the full length nature of one of the variants has not been defined.

References

Huang, C., et al. J. Exp. Clin. Cancer Res. 28, 11 (2009) :
Chen, Y., et al. Exp. Mol. Pathol. 82(3):256-261(2007)
Alli, Z., et al. Anticancer Res. 27 (1A), 373-377 (2007) :
Alli, Z., et al. Exp. Mol. Pathol. 81(3):245-248(2006)
Alli, Z., et al. Cell. Immunol. 237(2):141-146(2005)

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$ 365.00
$ 140.00
Cat# AP13575c
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