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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NSMAF Antibody (N-term)   

NSMAF Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NSMAF Antibody (N-term) AP13585a
    NSMAF Antibody (N-term) (Cat. #AP13585a) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the NSMAF antibody detected the NSMAF protein (arrow).
  • IHC-P - NSMAF Antibody (N-term) AP13585a
    NSMAF Antibody (N-term) (Cat. #AP13585a)immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NSMAF Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q92636
Other Accession NP_003571.2, NP_001138244.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 28-57 aa
Additional Information
Other Names Protein FAN, Factor associated with neutral sphingomyelinase activation, Factor associated with N-SMase activation, NSMAF, FAN
Target/Specificity This NSMAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-57 amino acids from the N-terminal region of human NSMAF.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNSMAF Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NSMAF
Synonyms FAN
Function Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N- smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE.
Tissue Location Ubiquitous. EMBL; X96586; CAA65405.1; -; mRNA EMBL; AK294009; BAG57371.1; -; mRNA EMBL; AC068522; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AC092700; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; CH471068; EAW86817.1; -; Genomic_DNA EMBL; BC041124; AAH41124.1; -; mRNA CCDS; CCDS47864.1; -. [Q92636-2] CCDS; CCDS6173.1; -. [Q92636-1] RefSeq; NP_001138244.1; NM_001144772.1. [Q92636-2] RefSeq; NP_003571.2; NM_003580.3. [Q92636-1] UniGene; Hs.372000; - ProteinModelPortal; Q92636; - SMR; Q92636; - BioGrid; 114019; 39 DIP; DIP-44106N; - IntAct; Q92636; 28 MINT; Q92636; - STRING; 9606.ENSP00000411012; - iPTMnet; Q92636; - PhosphoSitePlus; Q92636; - BioMuta; NSMAF; - DMDM; 209572614; - EPD; Q92636; - MaxQB; Q92636; - PaxDb; Q92636; - PeptideAtlas; Q92636; - PRIDE; Q92636; - ProteomicsDB; 75390; - ProteomicsDB; 75391; -. [Q92636-2] Ensembl; ENST00000038176; ENSP00000038176; ENSG00000035681. [Q92636-1] Ensembl; ENST00000427130; ENSP00000411012; ENSG00000035681. [Q92636-2] GeneID; 8439; - KEGG; hsa:8439; - UCSC; uc003xtt.4; human. [Q92636-1] CTD; 8439; - DisGeNET; 8439; - EuPathDB; HostDB:ENSG00000035681.7; - GeneCards; NSMAF; - H-InvDB; HIX0007527; - HGNC; HGNC:8017; NSMAF HPA; HPA023067; - HPA; HPA023148; - HPA; HPA023151; - MIM; 603043; gene neXtProt; NX_Q92636; - OpenTargets; ENSG00000035681; - PharmGKB; PA31795; - eggNOG; KOG1786; Eukaryota eggNOG; ENOG410XNQC; LUCA GeneTree; ENSGT00760000119083; - HOGENOM; HOG000231605; - HOVERGEN; HBG005639; - InParanoid; Q92636; - KO; K18953; - OMA; APYKIER; - OrthoDB; EOG091G01X8; - PhylomeDB; Q92636; - TreeFam; TF324912; - Reactome; R-HSA-5626978; TNFR1-mediated ceramide production SignaLink; Q92636; - ChiTaRS; NSMAF; human GenomeRNAi; 8439; - PRO; PR:Q92636; - Proteomes; UP000005640; Chromosome 8 Bgee; ENSG00000035681; - CleanEx; HS_NSMAF; - ExpressionAtlas; Q92636; baseline and differential Genevisible; Q92636; HS GO; GO:0005737; C:cytoplasm; TAS:ProtInc GO; GO:0005829; C:cytosol; TAS:Reactome GO; GO:0016230; F:sphingomyelin phosphodiesterase activator activity; IEA:Ensembl GO; GO:0006672; P:ceramide metabolic process; TAS:ProtInc GO; GO:0043065; P:positive regulation of apoptotic process; IEA:Ensembl GO; GO:2000304; P:positive regulation of ceramide biosynthetic process; TAS:Reactome GO; GO:0007165; P:signal transduction; TAS:ProtInc CDD; cd06071; Beach; 1 Gene3D; 1.10.1540.10; -; 1 Gene3D; 2.130.10.10; -; 3 InterPro; IPR000409; BEACH_dom InterPro; IPR036372; BEACH_dom_sf InterPro; IPR004182; GRAM InterPro; IPR023362; PH-BEACH_dom InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf InterPro; IPR001680; WD40_repeat InterPro; IPR017986; WD40_repeat_dom InterPro; IPR036322; WD40_repeat_dom_sf Pfam; PF02138; Beach; 1 Pfam; PF02893; GRAM; 1 Pfam; PF00400; WD40; 3 SMART; SM01026; Beach; 1 SMART; SM00568; GRAM; 1 SMART; SM00320; WD40; 7 SUPFAM; SSF50978; SSF50978; 1 SUPFAM; SSF81837; SSF81837; 1 PROSITE; PS50197; BEACH; 1 PROSITE; PS51783; PH_BEACH; 1 PROSITE; PS50082; WD_REPEATS_2; 4 PROSITE; PS50294; WD_REPEATS_REGION; 1 1: Evidence at protein level; Alternative splicing; Complete proteome; Polymorphism; Reference proteome; Repeat; WD repeat CHAIN 1 917 Protein FAN /FTId=PRO_0000050975 DOMAIN 176 247 GRAM DOMAIN 189 286 BEACH-type PH. {ECO:0000255|PROSITE- ProRule:PRU01119} DOMAIN 290 575 BEACH. {ECO:0000255|PROSITE- ProRule:PRU00026} REPEAT 628 658 WD 1 REPEAT 670 700 WD 2 REPEAT 712 740 WD 3 REPEAT 761 791 WD 4 REPEAT 803 833 WD 5 REPEAT 884 914 WD 6 COMPBIAS 23 28 Poly-Leu VAR_SEQ 1 20 MAFIRKKQQEQQLQLYSKER -> MTGQARFHRGAEACRAI RQCRSRRPRAAHPEGRAGSGRGSRHASPGVRRGG (in isoform 2) /FTId=VSP_042036 VARIANT 626 626 Y -> C (in dbSNP:rs2228505) /FTId=VAR_047023 CONFLICT 218 218 T -> A (in Ref. 2; BAG57371) SEQUENCE 917 AA; 104372 MW; 36498B5A1378E49C CRC64; MAFIRKKQQE QQLQLYSKER FSLLLLNLEE YYFEQHRANH ILHKGSHHER KIRGSLKICS KSVIFEPDSI SQPIIKIPLR DCIKIGKHGE NGANRHFTKA KSGGISLIFS QVYFIKEHNV VAPYKIERGK MEYVFELDVP GKVEDVVETL LQLHRASCLD KLGDQTAMIT AILQSRLART SFDKNRFQNI SEKLHMECKA EMVTPLVTNP GHVCITDTNL YFQPLNGYPK PVVQITLQDV RRIYKRRHGL MPLGLEVFCT EDDLCSDIYL KFYEPQDRDD LYFYIATYLE HHVAEHTAES YMLQWQRGHL SNYQYLLHLN NLADRSCNDL SQYPVFPWII HDYSSSELDL SNPGTFRDLS KPVGALNKER LERLLTRYQE MPEPKFMYGS HYSSPGYVLF YLVRIAPEYM LCLQNGRFDN ADRMFNSIAE TWKNCLDGAT DFKELIPEFY GDDVSFLVNS LKLDLGKRQG GQMVDDVELP PWASSPEDFL QKSKDALESN YVSEHLHEWI DLIFGYKQKG SDAVGAHNVF HPLTYEGGVD LNSIQDPDEK VAMLTQILEF GQTPKQLFVT PHPRRITPKF KSLSQTSSYN ASMADSPGEE SFEDLTEESK TLAWNNITKL QLHEHYKIHK EAVTGITVSR NGSSVFTTSQ DSTLKMFSKE SKMLQRSISF SNMALSSCLL LPGDATVITS SWDNNVYFYS IAFGRRQDTL MGHDDAVSKI CWHDNRLYSA SWDSTVKVWS GVPAEMPGTK RHHFDLLAEL EHDVSVDTIS LNAASTLLVS GTKEGTVNIW DLTTATLMHQ IPCHSGIVCD TAFSPDSRHV LSTGTDGCLN VIDVQTGMLI SSMTSDEPQR CFVWDGNSVL SGSQSGELLV WDLLGAKISE RIQGHTGAVT CIWMNEQCSS IITGGEDRQI IFWKLQY
Research Areas
Citations (0)

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Background

This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.

References

Bailey, S.D., et al. Diabetes Care (2010) In press :
Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Clarke, C.J., et al. Mol. Pharmacol. 74(4):1022-1032(2008)
Kolzer, M., et al. Biol. Chem. 385(12):1193-1195(2004)

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$ 295.00
$ 99.00
Cat# AP13585a
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