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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   UBE2W Antibody (C-term)   

UBE2W Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - UBE2W Antibody (C-term) AP13607b
    UBE2W Antibody (C-term) (Cat. #AP13607b) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the UBE2W antibody detected the UBE2W protein (arrow).
  • WB - UBE2W Antibody (C-term) AP13607b
    UBE2W Antibody (C-term) (Cat. #AP13607b) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the UBE2W antibody detected the UBE2W protein (arrow).
  • IHC-P - UBE2W Antibody (C-term) AP13607b
    UBE2W Antibody (C-term) (Cat. #AP13607b)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE2W Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q96B02
Other Accession B5DEI4, Q8VDW4, A6H795, Q1JPX4, NP_001001481.1, NP_060769.3
Reactivity Human, Mouse
Predicted Zebrafish, Bovine, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 17331 Da
Antigen Region 105-133 aa
Additional Information
Gene ID 55284
Other Names Ubiquitin-conjugating enzyme E2 W, N-terminus-conjugating E2, Ubiquitin carrier protein W, Ubiquitin-conjugating enzyme 16, UBC-16, Ubiquitin-protein ligase W, UBE2W, UBC16
Target/Specificity This UBE2W antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 105-133 amino acids from the C-terminal region of human UBE2W.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBE2W Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBE2W
Synonyms UBC16
Function Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3. After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Transfers ubiquitin in complex with RING/U-box type E3s that do not have active site cysteine residues to form thioester bonds with ubiquitin, and preferentially ubiquitinates the N-terminus of substrates, such as ATXN3, STUB1 and SUMO2.
Cellular Location Nucleus. Note=In the nucleus, colocalizes with FANCL
Tissue Location Widely expressed, with highest expression in brain, liver, pancreas and heart.
Research Areas
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Background

UBE2W accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and 'Lys-11'-linked polyubiquitination.

References

Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
Yin, G., et al. Front. Biosci. 11, 1500-1507 (2006) :

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$ 295.00
$ 99.00
Cat# AP13607b
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(40 western blots)
Availability: In Stock
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