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DOLPP1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DOLPP1 Antibody (C-term) AP13656b
    DOLPP1 Antibody (C-term) (Cat. #AP13656b) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the DOLPP1 antibody detected the DOLPP1 protein (arrow).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q86YN1
Other Accession Q9JMF7, NP_001129389.1, NP_065171.2
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 27031 Da
Antigen Region 207-236 aa
Additional Information
Gene ID 57171
Other Names Dolichyldiphosphatase 1, Dolichyl pyrophosphate phosphatase 1, DOLPP1, LSFR2
Target/Specificity This DOLPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-236 amino acids from the C-terminal region of human DOLPP1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDOLPP1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DOLPP1
Synonyms LSFR2
Function Required for efficient N-glycosylation. Necessary for maintaining optimal levels of dolichol-linked oligosaccharides. Hydrolyzes dolichyl pyrophosphate at a very high rate and dolichyl monophosphate at a much lower rate. Does not act on phosphatidate (By similarity).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Citations (0)

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Background

DOLPP1 is required for efficient N-glycosylation. Necessary for maintaining optimal levels of dolichol-linked oligosaccharides. Hydrolyzes dolichyl pyrophosphate at a very high rate and dolichyl monophosphate at a much lower rate. Does not act on phosphatidate (By similarity).

References

Humphray, S.J., et al. Nature 429(6990):369-374(2004)
Rush, J.S., et al. J. Biol. Chem. 277(47):45226-45234(2002)
Gilley, J., et al. Hum. Mol. Genet. 8(7):1313-1320(1999)
Wedgwood, J.F., et al. J. Biol. Chem. 255(3):1120-1123(1980)

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Cat# AP13656b
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(40 western blots)
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