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HIST1H1D Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - HIST1H1D Antibody (Center) AP13745c
    HIST1H1D Antibody (Center) (Cat. #AP13745c) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HIST1H1D antibody detected the HIST1H1D protein (arrow).
  • IHC-P - HIST1H1D Antibody (Center) AP13745c
    HIST1H1D Antibody (Center) (Cat. #AP13745c)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIST1H1D Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P16402
Other Accession NP_005311.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22350 Da
Antigen Region 135-164 aa
Additional Information
Gene ID 3007
Other Names Histone H13, Histone H1c, Histone H1s-2, HIST1H1D, H1F3
Target/Specificity This HIST1H1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the Central region of human HIST1H1D.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHIST1H1D Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms H1F3
Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Cellular Location Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in euchromatin. According to PubMed:10997781 is associated with inactive chromatin
Citations (0)

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Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.


Kim, J.J., et al. J. Hum. Genet. 55(1):27-31(2010)
Soranzo, N., et al. PLoS Genet. 5 (4), E1000445 (2009) :
Sovio, U., et al. PLoS Genet. 5 (3), E1000409 (2009) :
Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008)
Lettre, G., et al. Nat. Genet. 40(5):584-591(2008)

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Cat# AP13745c
(40 western blots)
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