|Application ||WB, IHC-P, E|
|Calculated MW||22350 Da|
|Antigen Region||135-164 aa|
|Other Names||Histone H13, Histone H1c, Histone H1s-2, HIST1H1D, H1F3|
|Target/Specificity||This HIST1H1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the Central region of human HIST1H1D.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||HIST1H1D Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).|
|Cellular Location||Nucleus. Chromosome. Note=According to PubMed:15911621 more commonly found in euchromatin. According to PubMed:10997781 is associated with inactive chromatin|
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Provided below are standard protocols that you may find useful for product applications.
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Kim, J.J., et al. J. Hum. Genet. 55(1):27-31(2010)
Soranzo, N., et al. PLoS Genet. 5 (4), E1000445 (2009) :
Sovio, U., et al. PLoS Genet. 5 (3), E1000409 (2009) :
Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008)
Lettre, G., et al. Nat. Genet. 40(5):584-591(2008)
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