|Application ||WB, E|
|Other Accession||P32577, P41241, P41239, Q0VBZ0, NP_001120662.1, NP_004374.1|
|Predicted||Bovine, Chicken, Mouse, Rat|
|Calculated MW||50704 Da|
|Antigen Region||33-61 aa|
|Other Names||Tyrosine-protein kinase CSK, C-Src kinase, Protein-tyrosine kinase CYL, CSK|
|Target/Specificity||This CSK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 33-61 amino acids from the N-terminal region of human CSK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CSK Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T- cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.|
|Cellular Location||Cytoplasm. Cell membrane. Note=Mainly cytoplasmic, also present in lipid rafts.|
|Tissue Location||Expressed in lung and macrophages.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
Niu, W., et al. Clin. Chim. Acta 411 (19-20), 1491-1495 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Mikkola, E.T., et al. J. Mol. Biol. 399(4):618-627(2010)
Takeuchi, F., et al. Circulation 121(21):2302-2309(2010)
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