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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PPP4C Antibody (Center)   

PPP4C Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PPP4C Antibody (Center) AP13756c
    PPP4C Antibody (Center) (Cat. #AP13756c) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPP4C antibody detected the PPP4C protein (arrow).
  • IHC-P - PPP4C Antibody (Center) AP13756c
    PPP4C Antibody (Center) (Cat. #AP13756c)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PPP4C Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P60510
Other Accession Q6IP91, Q5BJ92, P11084, P97470, A6H772, A9JRC7, A8WGP3, NP_002711.1
Reactivity Human
Predicted Zebrafish, Bovine, Mouse, Rabbit, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35080 Da
Antigen Region 160-189 aa
Additional Information
Gene ID 5531
Other Names Serine/threonine-protein phosphatase 4 catalytic subunit, PP4C, Pp4, Protein phosphatase X, PP-X, PPP4C, PPP4, PPX
Target/Specificity This PPP4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from the Central region of human PPP4C.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPPP4C Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPP4C
Synonyms PPP4, PPX
Function Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C- PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Research Areas
Citations (0)

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Background

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity).

References

Lee, D.H., et al. Nat. Struct. Mol. Biol. 17(3):365-372(2010)
Mourtada-Maarabouni, M., et al. Leuk. Res. 33(11):1539-1551(2009)
Kumar, R.A., et al. PLoS ONE 4 (2), E4582 (2009) :
Chen, G.I., et al. J. Biol. Chem. 283(43):29273-29284(2008)
Nakada, S., et al. EMBO Rep. 9(10):1019-1026(2008)

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$ 295.00
$ 99.00
Cat# AP13756c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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