|Application ||WB, IHC-P, E|
|Calculated MW||29263 Da|
|Antigen Region||70-98 aa|
|Other Names||Chymotrypsin-like elastase family member 3B, Elastase IIIB, Elastase-3B, Protease E, CELA3B, ELA3B|
|Target/Specificity||This CELA3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human CELA3B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CELA3B Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Efficient protease with alanine specificity but only little elastolytic activity.|
|Tissue Location||Pancreas. Not detectable in keratinocytes.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3B may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
Gao, J., et al. Oncol. Rep. 23(6):1683-1692(2010)
Teichmann, J., et al. Eur. J. Med. Res. 13(12):563-567(2008)
Hahn, J.U., et al. Pancreas 36(3):274-278(2008)
Mann, S.T., et al. Eur. J. Med. Res. 13(2):68-72(2008)
Vesterhus, M., et al. Diabetes Care 31(2):306-310(2008)
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