|Application ||WB, E|
|Other Accession||Q61686, NP_036249.1, NP_001120794.1, NP_001120793.1|
|Calculated MW||22225 Da|
|Antigen Region||88-117 aa|
|Other Names||Chromobox protein homolog 5, Antigen p25, Heterochromatin protein 1 homolog alpha, HP1 alpha, CBX5, HP1A|
|Target/Specificity||This CBX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 88-117 amino acids from the Central region of human CBX5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CBX5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.|
|Cellular Location||Nucleus. Chromosome. Chromosome, centromere Note=Colocalizes with HNRNPU in the nucleus (PubMed:19617346) Component of centromeric and pericentromeric heterochromatin Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Nozawa, R.S., et al. Nat. Cell Biol. 12(7):719-727(2010)
Zeng, W., et al. Epigenetics 5(4):287-292(2010)
Emelyanov, A.V., et al. J. Biol. Chem. 285(20):15027-15037(2010)
Kiyomitsu, T., et al. J. Cell Biol. 188(6):791-807(2010)
Chaturvedi, P., et al. PLoS ONE 5 (5), E10620 (2010) :
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