|Application ||WB, E|
|Calculated MW||49302 Da|
|Antigen Region||304-332 aa|
|Other Names||Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1, Protein SKD1, VPS4B, SKD1, VPS42|
|Target/Specificity||This VPS4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-332 amino acids from the C-terminal region of human VPS4B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||VPS4B Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).|
|Cellular Location||Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane; Peripheral membrane protein. Note=Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells|
|Tissue Location||Ubiquitously expressed.|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq].
Morita, E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(29):12889-12894(2010)
McDonough, C.W., et al. Hum. Genet. (2009) In press :
Bruce, E.A., et al. Virology 390(2):268-278(2009)
Inoue, M., et al. Traffic 9(12):2180-2189(2008)
Stuchell-Brereton, M.D., et al. Nature 449(7163):740-744(2007)
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