|Application ||WB, IF, E|
|Other Accession||Q9WUL0, Q04750, Q07050, NP_003277.1|
|Predicted||Hamster, Mouse, Rat|
|Calculated MW||90726 Da|
|Antigen Region||2-31 aa|
|Other Names||DNA topoisomerase 1, DNA topoisomerase I, TOP1|
|Target/Specificity||This TOP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human TOP1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TOP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.|
|Cellular Location||Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli|
|Tissue Location||Endothelial cells.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
Kjeldsen, E., et al. Anticancer Res. 30(9):3257-3265(2010)
Reinhold, W.C., et al. Cancer Res. 70(6):2191-2203(2010)
Tesauro, C., et al. Biochem. J. 425(3):531-539(2010)
Ballot, C., et al. Mol. Cancer Ther. 8(12):3307-3317(2009)
Lebedeva, N.A., et al. Biochemistry Mosc. 74(11):1278-1284(2009)
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