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TOP1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - TOP1 Antibody (N-term) AP13897a
    TOP1 Antibody (N-term) (Cat. #AP13897a) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TOP1 antibody detected the TOP1 protein (arrow).
  • IF - TOP1 Antibody (N-term) AP13897a
    Confocal immunofluorescent analysis of TOP1 Antibody (N-term)(Cat#AP13897a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P11387
Other Accession Q9WUL0, Q04750, Q07050, NP_003277.1
Reactivity Human
Predicted Hamster, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 90726 Da
Antigen Region 2-31 aa
Additional Information
Gene ID 7150
Other Names DNA topoisomerase 1, DNA topoisomerase I, TOP1
Target/Specificity This TOP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human TOP1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTOP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TOP1
Function Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.
Cellular Location Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Diffuse nuclear localization with some enrichment in nucleoli. On CPT treatment, cleared from nucleoli into nucleoplasm. Sumolyated forms found in both nucleoplasm and nucleoli
Tissue Location Endothelial cells.
Research Areas
Citations (0)

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This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.


Kjeldsen, E., et al. Anticancer Res. 30(9):3257-3265(2010)
Reinhold, W.C., et al. Cancer Res. 70(6):2191-2203(2010)
Tesauro, C., et al. Biochem. J. 425(3):531-539(2010)
Ballot, C., et al. Mol. Cancer Ther. 8(12):3307-3317(2009)
Lebedeva, N.A., et al. Biochemistry Mosc. 74(11):1278-1284(2009)

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$ 295.00
$ 99.00
Cat# AP13897a
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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