|Application ||WB, E|
|Calculated MW||56449 Da|
|Antigen Region||8-37 aa|
|Other Names||Neuronal acetylcholine receptor subunit alpha-7, CHRNA7, NACHRA7|
|Target/Specificity||This CHRNA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-37 amino acids from the N-terminal region of human CHRNA7.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CHRNA7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.|
|Cellular Location||Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Chernyavsky, A.I., et al. Am. J. Physiol., Cell Physiol. 299 (5), C903-C911 (2010) :
Saccone, N.L., et al. Genes Brain Behav. 9(7):741-750(2010)
Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010)
Jin, Y., et al. Int. J. Immunogenet. (2010) In press :
Schraufstatter, I.U., et al. J Stem Cells 4(4):203-215(2009)
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