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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Mouse Nek6 Antibody (C-term)   

Mouse Nek6 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Mouse Nek6 Antibody (C-term) AP13926b
    Mouse Nek6 Antibody (C-term) (Cat. #AP13926b) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Nek6 antibody detected the Nek6 protein (arrow).
  • WB - Mouse Nek6 Antibody (C-term) AP13926b
    Western blot analysis of Nek6 (arrow) using rabbit polyclonal Mouse Nek6 Antibody (C-term) (Cat. #AP13926b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek6 gene.
  • IHC-P - Mouse Nek6 Antibody (C-term) AP13926b
    Mouse Nek6 Antibody (C-term) (AP13926b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Nek6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9ES70
Other Accession P59895, A2BD05, Q9HC98, NP_001153103.1, NP_067619.1
Reactivity Human, Mouse
Predicted Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35742 Da
Antigen Region 251-280 aa
Additional Information
Gene ID 59126
Other Names Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Nek6
Target/Specificity This Mouse Nek6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-280 amino acids from the C-terminal region of mouse Nek6.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Nek6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Nek6
Function Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, TRIP4, RPS6KB1 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (By similarity). Phosphorylates STAT3.
Cellular Location Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Co- localizes with APBB1 at the nuclear speckles. Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis (By similarity).
Research Areas
Citations (0)

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Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis (By similarity).


Jeon, Y.J., et al. J. Biol. Chem. 285(36):28126-28133(2010)
Kaput, J., et al. Physiol. Genomics 18(3):316-324(2004)
Visel, A., et al. Nucleic Acids Res. 32 (DATABASE ISSUE), D552-D556 (2004) :
Hashimoto, Y., et al. Biochem. Biophys. Res. Commun. 293(2):753-758(2002)
Feige, E., et al. Mech. Dev. 110 (1-2), 219-223 (2002) :

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Cat# AP13926b
(40 western blots)
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