|Application ||WB, IHC-P, E|
|Other Accession||P59895, A2BD05, Q9HC98, NP_001153103.1, NP_067619.1|
|Calculated MW||35742 Da|
|Antigen Region||251-280 aa|
|Other Names||Serine/threonine-protein kinase Nek6, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Nek6|
|Target/Specificity||This Mouse Nek6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-280 amino acids from the C-terminal region of mouse Nek6.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse Nek6 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, TRIP4, RPS6KB1 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence (By similarity). Phosphorylates STAT3.|
|Cellular Location||Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Co- localizes with APBB1 at the nuclear speckles. Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
Activated during M phase. Required for chromosome segregation at metaphase-anaphase transition and therefore for mitotic progression. Inhibition of activity results in apoptosis (By similarity).
Jeon, Y.J., et al. J. Biol. Chem. 285(36):28126-28133(2010)
Kaput, J., et al. Physiol. Genomics 18(3):316-324(2004)
Visel, A., et al. Nucleic Acids Res. 32 (DATABASE ISSUE), D552-D556 (2004) :
Hashimoto, Y., et al. Biochem. Biophys. Res. Commun. 293(2):753-758(2002)
Feige, E., et al. Mech. Dev. 110 (1-2), 219-223 (2002) :
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