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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   Mouse Nek8 Antibody (C-term)   

Mouse Nek8 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Mouse Nek8 Antibody (C-term) AP13927B
    Mouse Nek8 Antibody (C-term) (Cat. #AP13927b) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the Nek8 antibody detected the Nek8 protein (arrow).
  • WB - Mouse Nek8 Antibody (C-term) AP13927B
    Western blot analysis of Nek8 (arrow) using rabbit polyclonal Mouse Nek8 Antibody (C-term) (Cat. #AP13927b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Nek8 gene.
  • WB - Mouse Nek8 Antibody (C-term) AP13927B
    Mouse Nek8 Antibody (C-term) (Cat. #AP13927b) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Nek8 antibody detected the Nek8 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q91ZR4
Other Accession NP_543125.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 75265 Da
Antigen Region 519-547 aa
Additional Information
Gene ID 140859
Other Names Serine/threonine-protein kinase Nek8, Never in mitosis A-related kinase 8, NimA-related protein kinase 8, Nek8, Jck
Target/Specificity This Mouse Nek8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the C-terminal region of mouse Nek8.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Nek8 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Nek8
Synonyms Jck
Function Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cell projection, cilium Note=Predominantly cytoplasmic. Localizes to the proximal region of the primary cilium and is not observed in dividing cells
Tissue Location Kidney, liver, and testis.
Research Areas
Citations (0)

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This gene encodes a NIMA-related kinase. Members of this serine/threonine protein kinase family are structurally-related to NIMA (never in mitosis, gene A) which controls mitotic signaling in Aspergillus nidulans.


Hellman, N.E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(43):18499-18504(2010)
Ahmadie, R., et al. J. Nutr. 140(8):1438-1444(2010)
Natoli, T.A., et al. Nat. Med. 16(7):788-792(2010)
Shiba, D., et al. Cytoskeleton (Hoboken) 67(2):112-119(2010)
Sohara, E., et al. J. Am. Soc. Nephrol. 19(3):469-476(2008)

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$ 295.00
$ 99.00
Cat# AP13927B
(40 western blots)
Availability: In Stock
Bulk Size
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