|Application ||WB, IHC-P, E|
|Other Accession||Q63604, NP_001020245.1, NP_032771.1|
|Calculated MW||92133 Da|
|Antigen Region||343-372 aa|
|Other Names||BDNF/NT-3 growth factors receptor, GP145-TrkB/GP95-TrkB, Trk-B, Neurotrophic tyrosine kinase receptor type 2, TrkB tyrosine kinase, Ntrk2, Trkb|
|Target/Specificity||This Mouse Ntrk2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-372 amino acids from the Central region of mouse Ntrk2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse Ntrk2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin- 4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. Isoform GP95-TRKB may also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein Endosome membrane; Single-pass type I membrane protein Note=Internalized to endosomes upon ligand-binding|
|Tissue Location||Widely expressed in the central and peripheral nervous system. The different forms are differentially expressed in various cell types. Isoform GP95-TRKB is specifically expressed in glial cells|
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Provided below are standard protocols that you may find useful for product applications.
Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
Xu, X., et al. Proc. Natl. Acad. Sci. U.S.A. 107(44):19126-19131(2010)
Lobo, M.K., et al. Science 330(6002):385-390(2010)
Gascon, E., et al. J. Neurosci. 30(37):12414-12423(2010)
Nikoletopoulou, V., et al. Nature 467(7311):59-63(2010)
Ohnishi, H., et al. J. Neurosci. 30(31):10472-10483(2010)
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