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ACK1 Antibody (Center Y284)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP13936c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP13936c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
ACK1 Antibody (Center Y284) - Product info | |
| Application | WB
|
| Primary Accession | Q07912 |
| Other Accession | NP_005772.3, NP_001010938.1 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 114569 Da |
ACK1 Antibody (Center Y284) - Additional info | |
| Gene ID 10188 | |
| Other Names TNK2; ACK1; Activated CDC42 kinase 1; Tyrosine kinase non-receptor protein 2 | |
| Target/Specificity This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human ACK1. | |
| Dilution WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ACK1 Antibody (Center Y284) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ACK1 Antibody (Center Y284) - Protein Information | |
| Name TNK2 | |
| Synonyms ACK1 | |
| Function Non-receptor tyrosine-protein and serine/threonine- protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr- 287'. Phosphorylates MCF2, thereby enhancing its activity as a guanine nucleotide exchange factor (GEF) toward Rho family proteins. Contributes to the control of AXL receptor levels Confers metastatic properties on cancer cells and promotes tumor growth by negatively regulating tumor suppressor such as WWOX and positively regulating pro-survival factors such as AKT1 and AR | |
| Cellular Location Cell membrane. Nucleus. Endosome. Cell junction, adherens junction (By similarity). Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Cytoplasmic vesicle, clathrin-coated vesicle. Membrane, clathrin- coated pit. Note=The Tyr-284 phosphorylated form is found both in the membrane and nucleus. Co-localizes with EGFR on endosomes Nuclear translocation is CDC42-dependent | |
| Tissue Location The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages | |
ACK1 Antibody (Center Y284) - Related products
AP13936c: ACK1 Antibody (Center Y284)
AP3587a: Phospho-ACK1-pY518 Antibody
AP3702a: Phospho-ACK1- Y826 Antibody
AP7696a: ACK1 Antibody (N-term)
AP7696b: ACK1 Antibody (C-term)
AP7696c: ACK1 Antibody (Center)
AP7696d: ACK1 Antibody (C-term K992)
DC05789: Human TNK2 cDNA Clone
LY10004a: TNK2 Over-expression Lysate
BP3587a: Phospho-ACK1-pY518 Antibody Blocking Peptide
BP3702a: Phospho-ACK1-Y826 Antibody Blocking Peptide
BP7696a: ACK1 Antibody (N-term) Blocking Peptide
BP7696b: ACK1 Antibody (C-term) Blocking Peptide
BP7696c: ACK1 Antibody (Center) Blocking Peptide
ACK1 Antibody (Center Y284) - Application data
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ACK1 Antibody (Center Y284) (Cat. #AP13936c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ACK1 antibody detected the ACK1 protein (arrow).
ACK1 Antibody (Center Y284) - Research Areas
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BACKGROUND
This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
REFERENCES
Mahajan, K., et al. J. Cell. Physiol. 224(2):327-333(2010) Chua, B.T., et al. Mol Oncol 4(4):323-334(2010) Prieto-Echague, V., et al. J. Biol. Chem. 285(14):10605-10615(2010) Lin, Q., et al. Mol. Cell. Biol. 30(6):1541-1554(2010) Pao-Chun, L., et al. J. Biol. Chem. 284(50):34954-34963(2009)
