|Application ||WB, IHC-P, E|
|Other Accession||NP_003048.1, NP_694857.1|
|Calculated MW||61154 Da|
|Antigen Region||511-539 aa|
|Other Names||Solute carrier family 22 member 1, Organic cation transporter 1, hOCT1, SLC22A1, OCT1|
|Target/Specificity||This SLC22A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 511-539 amino acids from the C-terminal region of human SLC22A1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SLC22A1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)- N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin- dependent kinase II and LCK tyrosine kinase.|
|Cellular Location||Basolateral cell membrane; Multi-pass membrane protein|
|Tissue Location||Widely expressed with high level in liver. Isoform 1 and isoform 2 are expressed in liver. Isoform 1, isoform 2, isoform 3 and isoform 4 are expressed in glial cell lines|
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Provided below are standard protocols that you may find useful for product applications.
Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. Two transcript variants encoding two different isoforms have been found for this gene, but only the longer variant encodes a functional transporter. [provided by RefSeq].
Takahashi, N., et al. J. Hum. Genet. 55(11):731-737(2010)
Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010)
Hu, M., et al. Pharmacogenet. Genomics 20(10):634-637(2010)
Gambineri, A., et al. J. Clin. Endocrinol. Metab. 95 (10), E204-E208 (2010) :
Becker, M.L., et al. Neurogenetics (2010) In press :
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