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C4B Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - C4B Antibody (Center) AP13985c
    Anti-C4 Antibody (Center) at 1:2000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 193 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P0C0L4
Other Accession P0C0L5, NP_001002029.3
Reactivity Human
Predicted Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 192785 Da
Antigen Region 655-684 aa
Additional Information
Gene ID 720;721
Other Names Complement C4-A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, Complement C4 beta chain, Complement C4-A alpha chain, C4a anaphylatoxin, C4b-A, C4d-A, Complement C4 gamma chain, C4A, CO4, CPAMD2
Target/Specificity This C4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 655-684 amino acids from the Central region of human C4B.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsC4B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name C4A
Synonyms CO4, CPAMD2
Function Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.
Cellular Location Secreted.
Tissue Location Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland,and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extra-hepatic sites of expression may be important for the local protection and inflammatory response
Research Areas
Citations (0)

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This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.


Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Fernando, M.M., et al. Hum. Mutat. 31(7):866-874(2010)
Mostafa, G.A., et al. J. Neuroimmunol. 223 (1-2), 115-119 (2010) :
Hamad, O.A., et al. J. Immunol. 184(5):2686-2692(2010)
Mougey, R. Immunohematology 26(1):30-38(2010)

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Cat# AP13985c
(40 western blots)
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