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RFX5 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | P48382 |
| Other Accession | NP_001020774.1, NP_000440.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 65323 Da |
| Antigen Region | 237-266 aa |
| Gene ID | 5993 |
|---|---|
| Other Names | DNA-binding protein RFX5, Regulatory factor X 5, RFX5 |
| Target/Specificity | This RFX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-266 amino acids from the Central region of human RFX5. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | RFX5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RFX5 |
|---|---|
| Function | Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters. |
| Cellular Location | Nucleus. |
| Tissue Location | Ubiquitous. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq].
References
Laird, K.M., et al. J. Mol. Biol. 403(1):40-51(2010)
Kong, X., et al. J. Mol. Cell. Cardiol. 46(3):292-299(2009)
Garvie, C.W., et al. Biochim. Biophys. Acta 1779(12):797-804(2008)
Ennis, S., et al. Lancet 372(9652):1828-1834(2008)
Xu, Y., et al. J. Biol. Chem. 282(36):26046-26056(2007)
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