|Application ||WB, E|
|Other Accession||NP_001020774.1, NP_000440.1|
|Calculated MW||65323 Da|
|Antigen Region||237-266 aa|
|Other Names||DNA-binding protein RFX5, Regulatory factor X 5, RFX5|
|Target/Specificity||This RFX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-266 amino acids from the Central region of human RFX5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RFX5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.|
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Provided below are standard protocols that you may find useful for product applications.
A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq].
Laird, K.M., et al. J. Mol. Biol. 403(1):40-51(2010)
Kong, X., et al. J. Mol. Cell. Cardiol. 46(3):292-299(2009)
Garvie, C.W., et al. Biochim. Biophys. Acta 1779(12):797-804(2008)
Ennis, S., et al. Lancet 372(9652):1828-1834(2008)
Xu, Y., et al. J. Biol. Chem. 282(36):26046-26056(2007)
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