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RFX5 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - RFX5 Antibody (Center) AP14004c
    RFX5 Antibody (Center) (Cat. #AP14004c) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the RFX5 antibody detected the RFX5 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P48382
Other Accession NP_001020774.1, NP_000440.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 65323 Da
Antigen Region 237-266 aa
Additional Information
Gene ID 5993
Other Names DNA-binding protein RFX5, Regulatory factor X 5, RFX5
Target/Specificity This RFX5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-266 amino acids from the Central region of human RFX5.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRFX5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RFX5
Function Activates transcription from class II MHC promoters. Recognizes X-boxes. Mediates cooperative binding between RFX and NF-Y. RFX binds the X1 box of MHC-II promoters.
Cellular Location Nucleus.
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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A lack of MHC-II expression results in a severe immunodeficiency syndrome called MHC-II deficiency, or the bare lymphocyte syndrome (BLS; MIM 209920). At least 4 complementation groups have been identified in B-cell lines established from patients with BLS. The molecular defects in complementation groups B, C, and D all lead to a deficiency in RFX, a nuclear protein complex that binds to the X box of MHC-II promoters. The lack of RFX binding activity in complementation group C results from mutations in the RFX5 gene encoding the 75-kD subunit of RFX (Steimle et al., 1995). RFX5 is the fifth member of the growing family of DNA-binding proteins sharing a novel and highly characteristic DNA-binding domain called the RFX motif. Multiple alternatively spliced transcript variants have been found but the full-length natures of only two have been determined. [provided by RefSeq].


Laird, K.M., et al. J. Mol. Biol. 403(1):40-51(2010)
Kong, X., et al. J. Mol. Cell. Cardiol. 46(3):292-299(2009)
Garvie, C.W., et al. Biochim. Biophys. Acta 1779(12):797-804(2008)
Ennis, S., et al. Lancet 372(9652):1828-1834(2008)
Xu, Y., et al. J. Biol. Chem. 282(36):26046-26056(2007)

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$ 295.00
$ 99.00
Cat# AP14004c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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