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CELA2A Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CELA2A Antibody (C-term) AP14005b
    Anti-CELA2A Antibody (C-term) at 1:1000 dilution + human pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CELA2A Antibody (C-term) AP14005b
    Anti-CELA2A Antibody (C-term) at 1:1000 dilution + human pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - CELA2A Antibody (C-term) AP14005b
    Anti-CELA2A Antibody (C-term) at 1:1000 dilution + human pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • SPECIFICATION
  • CITATIONS: 2
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P08217
Other Accession P08419, NP_254275.1
Reactivity Human
Predicted Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 28888 Da
Antigen Region 231-260 aa
Additional Information
Gene ID 63036
Other Names Chymotrypsin-like elastase family member 2A, Elastase-2A, CELA2A, ELA2A
Target/Specificity This CELA2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-260 amino acids from the C-terminal region of human CELA2A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCELA2A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CELA2A
Synonyms ELA2A
Function Acts upon elastin.
Cellular Location Secreted.
Tissue Location Pancreas. Not detected in keratinocytes.
Citations ( 0 )

Background

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human elastases, elastase 2A is secreted from the pancreas as a zymogen. In other species, elastase 2A has been shown to preferentially cleave proteins after leucine, methionine, and phenylalanine residues.

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Lausen, J., et al. Oncogene 25(9):1349-1357(2006)
Szepessy, E., et al. Pancreatology 6 (1-2), 117-122 (2006) :

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$ 295.00
$ 99.00
Cat# AP14005b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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