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CYB5A Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CYB5A Antibody (Center) AP14033c
    CYB5A Antibody (Center) (Cat. #AP14033c) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CYB5A antibody detected the CYB5A protein (arrow).
  • WB - CYB5A Antibody (Center) AP14033c
    Western blot analysis of CYB5A (arrow) using rabbit polyclonal CYB5A Antibody (Center) (Cat. #AP14033c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CYB5A gene.
  • IHC-P - CYB5A Antibody (Center) AP14033c
    CYB5A Antibody (Center) (AP14033c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CYB5A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P00167
Other Accession NP_001905.1, NP_001177736.1, NP_683725.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 15330 Da
Antigen Region 69-98 aa
Additional Information
Gene ID 1528
Other Names Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5
Target/Specificity This CYB5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the Central region of human CYB5A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYB5A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYB5A
Synonyms CYB5
Function Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.
Cellular Location Isoform 1: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side
Research Areas
Citations (0)

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The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene.


Gardner, A.M., et al. J. Biol. Chem. 285(31):23850-23857(2010)
Kok, R.C., et al. J. Clin. Endocrinol. Metab. 95(3):994-999(2010)
Sacco, J.C., et al. Pharmacogenet. Genomics 20(1):26-37(2010)
Maghzal, G.J., et al. J. Biol. Chem. 283(18):12014-12025(2008)
Inui, H., et al. Biochemistry 46(35):10213-10221(2007)

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$ 295.00
$ 99.00
Cat# AP14033c
(40 western blots)
Availability: In Stock
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