|Application ||WB, IHC-P, E|
|Other Accession||NP_001905.1, NP_001177736.1, NP_683725.1|
|Calculated MW||15330 Da|
|Antigen Region||69-98 aa|
|Other Names||Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5|
|Target/Specificity||This CYB5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 69-98 amino acids from the Central region of human CYB5A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CYB5A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases.|
|Cellular Location||Isoform 1: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene.
Gardner, A.M., et al. J. Biol. Chem. 285(31):23850-23857(2010)
Kok, R.C., et al. J. Clin. Endocrinol. Metab. 95(3):994-999(2010)
Sacco, J.C., et al. Pharmacogenet. Genomics 20(1):26-37(2010)
Maghzal, G.J., et al. J. Biol. Chem. 283(18):12014-12025(2008)
Inui, H., et al. Biochemistry 46(35):10213-10221(2007)
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