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DDX4 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - DDX4 Antibody (C-term) AP1403b
    Anti-DDX4 Antibody (C-term) at 1:1000 dilution + human ovary lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 79 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - DDX4 Antibody (C-term) AP1403b
    Formalin-fixed and paraffin-embedded human testis tissue reacted with DDX4 antibody (C-term) (Cat.#AP1403b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NQI0
Other Accession Q64060, Q6GWX0, Q61496, Q4R5S7, Q5W5U4
Reactivity Human
Predicted Bovine, Monkey, Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 79308 Da
Antigen Region 554-583 aa
Additional Information
Gene ID 54514
Other Names Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA
Target/Specificity This DDX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 554-583 amino acids from the C-terminal region of human DDX4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDDX4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DDX4
Synonyms VASA
Function May play a role in germ cell development. May play a role in sperm motility.
Cellular Location Cytoplasm. Cytoplasm, perinuclear region
Tissue Location Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.
Research Areas
Citations (0)

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DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.


Guo,X., Asian J. Androl. 9 (3), 339-344 (2007)
Zeeman,A.M., Lab. Invest. 82 (2), 159-166 (2002)
Castrillon,D.H., Proc. Natl. Acad. Sci. U.S.A. 97 (17), 9585-9590 (2000)

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$ 295.00
$ 99.00
Cat# AP1403b
(40 western blots)
Availability: In Stock
Bulk Size
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