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UBP1 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - UBP1 Antibody (Center) AP14065c
    Fluorescent image of A549 cell stained with UBP1 Antibody (Center)(Cat#AP14065c).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UBP1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).UBP1 immunoreactivity is localized to Nucleus significantly.
  • WB - UBP1 Antibody (Center) AP14065c
    UBP1 Antibody (Center) (Cat. #AP14065c) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UBP1 antibody detected the UBP1 protein (arrow).
  • IHC-P - UBP1 Antibody (Center) AP14065c
    UBP1 Antibody (Center) (AP14065c)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, IF, WB, E
Primary Accession Q9NZI7
Other Accession Q811S7, NP_001121632.1, NP_055332.3, NP_001121633.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 60491 Da
Antigen Region 266-295 aa
Additional Information
Gene ID 7342
Other Names Upstream-binding protein 1, Transcription factor LBP-1, UBP1, LBP1
Target/Specificity This UBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-295 amino acids from the Central region of human UBP1.
Dilution IF~~1:10~50
WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUBP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UBP1
Synonyms LBP1
Function Functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2 (By similarity). Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.
Cellular Location Nucleus.
Tissue Location Expressed in adrenal tissue, JEG-3, NCI-H295A, Hep-G2 and HeLa cell lines.
Citations (0)

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Background

UBP1 functions as a transcriptional activator in a promoter context-dependent manner. Modulates the placental expression of CYP11A1. Involved in regulation of the alpha-globin gene in erythroid cells. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with TFCP2 (By similarity). Involved in regulation of the alpha-globin gene in erythroid cells. Binds strongly to sequences around the HIV-1 initiation site and weakly over the TATA-box. Represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA-box.

References

Katsura, A., et al. Genes Cells 14(10):1183-1196(2009)
Koutnikova, H., et al. PLoS Genet. 5 (8), E1000591 (2009) :
Henderson, Y.C., et al. DNA Cell Biol. 27(2):71-79(2008)
Huang, N., et al. Mol. Endocrinol. 19(2):409-420(2005)
Huang, N., et al. J. Biol. Chem. 275(4):2852-2858(2000)

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Cat# AP14065c
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(40 western blots)
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