|Application ||WB, IHC-P, E|
|Other Accession||Q64536, Q15119, NP_598428.2|
|Calculated MW||46041 Da|
|Antigen Region||78-107 aa|
|Other Names||[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, Pdk2|
|Target/Specificity||This Mouse Pdk2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-107 amino acids from the N-terminal region of mouse Pdk2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Mouse Pdk2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.|
|Cellular Location||Mitochondrion matrix|
|Tissue Location||Detected in heart (at protein level).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Pdk2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Sun, W., et al. Clin. Cancer Res. 15(2):476-484(2009)
Pagliarini, D.J., et al. Cell 134(1):112-123(2008)
Osafune, K., et al. Development 133(1):151-161(2006)
Papin, J., et al. Curr. Opin. Biotechnol. 15(1):78-81(2004)
Mootha, V.K., et al. Cell 115(5):629-640(2003)
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