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Nanos Homologue 1 (NANOS1) Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP1407b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP1407b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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- Citiations : 0
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Nanos Homologue 1 (NANOS1) Antibody (C-term) - Product info | |
| Application | WB, IHC, IF, FC
|
| Primary Accession | Q8WY41 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 30230 Da |
Nanos Homologue 1 (NANOS1) Antibody (C-term) - Additional info | |
| Gene ID 340719 | |
| Other Names NANOS1; NOS1; Nanos homolog 1; EC_Rep1a | |
| Target/Specificity This NANOS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263~292 amino acids from the C-terminal region of human NANOS1. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:10~50 IF~~1:10~50 FC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Nanos Homologue 1 (NANOS1) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Nanos Homologue 1 (NANOS1) Antibody (C-term) - Protein Information | |
| Name NANOS1 | |
| Synonyms NOS1 | |
| Function May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin Up-regulates the production of MMP14 to promote tumor cell invasion | |
| Cellular Location Cytoplasm, perinuclear region. Cytoplasm. Note=Co-localizes with SNAPIN and PUM2 in the perinuclear region of germ cells | |
| Tissue Location Testis and ovary (at protein level). Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness | |
Nanos Homologue 1 (NANOS1) Antibody (C-term) - Related products
AP1407b: Nanos Homologue 1 (NANOS1) Antibody (C-term)
RI13489: NANOS1 predesign siRNA
Nanos Homologue 1 (NANOS1) Antibody (C-term) - Application data
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Western blot analysis of anti-NANOS1 Antibody (C-term) (RB13839) in T47D cell line lysates (35ug/lane). NANOS1 (arrow) was detected using the purified Pab (1:60 dilution).
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with NANOS1 antibody (C-term) (Cat.#AP1407b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Immunofluorescence analysis of anti-NANOS1 Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
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Flow cytometric analysis of hela cells using Nanos Homologue 1 (NANOS1) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Nanos Homologue 1 (NANOS1) Antibody (C-term) - Research Areas
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BACKGROUND
NANOS1 may regulate translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets.
REFERENCES
Strumane,K., Cancer Res. 66 (20), 10007-10015 (2006) Kurokawa,H., Dev. Growth Differ. 48 (3), 209-221 (2006)
