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>   home   >   Products   >   Primary Antibodies   >   IGF1 Antibody (C-term)   

IGF1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - IGF1 Antibody (C-term) AP14099b
    IGF1 Antibody (C-term) (Cat. #AP14099b) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the IGF1 antibody detected the IGF1 protein (arrow).
  • IF - IGF1 Antibody (C-term) AP14099b
    Confocal immunofluorescent analysis of IGF1 Antibody (C-term)(Cat#AP14099b) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • FC - IGF1 Antibody (C-term) AP14099b
    IGF1 Antibody (C-term) (Cat. #AP14099b) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IHC-P - IGF1 Antibody (C-term) AP14099b
    IGF1 Antibody (C-term) (Cat. #AP14099b)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • IHC-P - IGF1 Antibody (C-term) AP14099b
    IGF1 Antibody (C-term) (Cat. #AP14099b)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, FC, IHC-P, E
Primary Accession P05019-2
Other Accession P01343, NP_000609.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 115-144 aa
Additional Information
Other Names IGF1; IBP1; Insulin-like growth factor I; Mechano growth factor; Somatomedin-C
Target/Specificity This IGF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-144 amino acids from the C-terminal region of human IGF1.
Dilution WB~~1:1000
IF~~1:10~50
FC~~1:10~50
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsIGF1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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Background

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.

References

Li, M., et al. J. Biol. Chem. 285(40):30480-30488(2010)
Canzian, F., et al. Hum. Mol. Genet. 19(19):3873-3884(2010)
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Szczesny, G., et al. Arch Orthop Trauma Surg (2010) In press :

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Cat# AP14099b
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(40 western blots)
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