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NCK2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NCK2 Antibody (N-term) AP14107a
    NCK2 Antibody (N-term) (Cat. #AP14107a) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the NCK2 antibody detected the NCK2 protein (arrow).
  • IHC-P - NCK2 Antibody (N-term) AP14107a
    NCK2 Antibody (N-term) (AP14107a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NCK2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43639
Other Accession NP_001004720.1, NP_003572.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 42915 Da
Antigen Region 63-92 aa
Additional Information
Gene ID 8440
Other Names Cytoplasmic protein NCK2, Growth factor receptor-bound protein 4, NCK adaptor protein 2, Nck-2, SH2/SH3 adaptor protein NCK-beta, NCK2, GRB4
Target/Specificity This NCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human NCK2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNCK2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCK2
Synonyms GRB4
Function Adapter protein which associates with tyrosine- phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1- dependent transcriptional activation in response to activated Ras signaling.
Cellular Location Cytoplasm. Endoplasmic reticulum
Tissue Location Ubiquitous.
Research Areas
Citations (0)

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This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.


Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Guan, S., et al. J. Invest. Dermatol. 129(8):1909-1920(2009)
Liu, J., et al. PLoS ONE 4 (11), E7805 (2009) :
Akiyama, M., et al. Br J Ophthalmol 92(9):1293-1296(2008)
Park, S., et al. J. Biomol. NMR 34(3):203-208(2006)

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$ 295.00
$ 99.00
Cat# AP14107a
(40 western blots)
Availability: In Stock
Bulk Size
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