|Application ||WB, IHC-P, E|
|Other Accession||NP_001004720.1, NP_003572.2|
|Calculated MW||42915 Da|
|Antigen Region||63-92 aa|
|Other Names||Cytoplasmic protein NCK2, Growth factor receptor-bound protein 4, NCK adaptor protein 2, Nck-2, SH2/SH3 adaptor protein NCK-beta, NCK2, GRB4|
|Target/Specificity||This NCK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human NCK2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NCK2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Adapter protein which associates with tyrosine- phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1- dependent transcriptional activation in response to activated Ras signaling.|
|Cellular Location||Cytoplasm. Endoplasmic reticulum|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Guan, S., et al. J. Invest. Dermatol. 129(8):1909-1920(2009)
Liu, J., et al. PLoS ONE 4 (11), E7805 (2009) :
Akiyama, M., et al. Br J Ophthalmol 92(9):1293-1296(2008)
Park, S., et al. J. Biomol. NMR 34(3):203-208(2006)
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