|Application ||WB, IHC-P, E|
|Other Accession||Q5G6V9, P45591, Q148F1, NP_619579.1, NP_068733.1|
|Predicted||Bovine, Mouse, Pig|
|Calculated MW||18737 Da|
|Antigen Region||25-54 aa|
|Other Names||Cofilin-2, Cofilin, muscle isoform, CFL2|
|Target/Specificity||This CFL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human CFL2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CFL2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. Its F-actin depolymerization activity is regulated by association with CSPR3 (PubMed:19752190). It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods. Required for muscle maintenance. May play a role during the exchange of alpha-actin forms during the early postnatal remodeling of the sarcomere (By similarity).|
|Cellular Location||Nucleus matrix. Cytoplasm, cytoskeleton. Note=Colocalizes with CSPR3 in the Z line of sarcomeres.|
|Tissue Location||Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants.
Vogel, S., et al. J. Biol. Chem. 285(44):33756-33763(2010) Borensztajn, K., et al. Thromb. Res. 125 (6), E323-E328 (2010) : Papalouka, V., et al. Mol. Cell. Biol. 29(22):6046-6058(2009) Wu, Y., et al. Retrovirology 5, 95 (2008) : Agrawal, P.B., et al. Am. J. Hum. Genet. 80(1):162-167(2007)
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