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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Mouse Scyl2 Antibody (Center)   

Mouse Scyl2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Mouse Scyl2 Antibody (Center) AP14165c
    Anti-Mouse Scyl2 Antibody (Center) at 1:1000 dilution + mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 103 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Mouse Scyl2 Antibody (Center) AP14165c
    Mouse Scyl2 Antibody (Center) (Cat. #AP14165c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Scyl2 antibody detected the Scyl2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q8CFE4
Other Accession NP_932138.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 103317 Da
Antigen Region 585-613 aa
Additional Information
Gene ID 213326
Other Names SCY1-like protein 2, Coated vesicle-associated kinase of 104 kDa, Scyl2, Cvak104, D10Ertd802e
Target/Specificity This Mouse Scyl2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 585-613 amino acids from the Central region of mouse Scyl2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMouse Scyl2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Scyl2
Synonyms Cvak104, D10Ertd802e
Function Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. May be a serine/threonine-protein kinase. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system (By similarity).
Cellular Location Cytoplasm, perinuclear region. Cytoplasmic vesicle, clathrin-coated vesicle. Golgi apparatus, trans-Golgi network membrane. Endosome membrane. Note=Plasma membrane-associated in clathrin-coated vesicles. Colocalizes to the trans-Golgi network (TGN) and to endosomal membranes with clathrin, transferrin and plasma membrane adapter AP1 and AP3 complexes (By similarity)
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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Background

Component of AP2-containing clathrin coated structures at the plasma membrane or of endocytic coated vesicles. Scyl2 may be a serine/threonine-protein kinase. May regulate clathrin-dependent trafficking between the TGN and/or the endosomal system (By similarity).

References

Duwel, M., et al. Mol. Biol. Cell 17(10):4513-4525(2006)
Zambrowicz, B.P., et al. Proc. Natl. Acad. Sci. U.S.A. 100(24):14109-14114(2003)
Stryke, D., et al. Nucleic Acids Res. 31(1):278-281(2003)

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$ 295.00
$ 99.00
Cat# AP14165c
Size:
Quantity:
(40 western blots)
Availability: In Stock
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